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Working on Application of Supercritical Fluid Chromatography for Chiral separation and transferring method on Preparative scale for purification of Chiral Molecules. Supporting for Method development and purification support for chiral separations at Piramal Discovery solutions.
April 2020 - present
Piramal Pharma Solutions
- Research scientist
- Method development and transfer method for purification of Chiral Molecules with using Chromatographic system
May 2016 - April 2020
Piramal Enterprises Ltd.
- Senior Research Associate
- Working on Method development, separation and isolation of Chiral molecules with using Supercritical Fluid Chromatography
The work describes a novel supercritical fluid chromatography method for the separation of four stereoisomers, RZ(+), SZ(-), RE(+) and SE(-) of luliconazole, an antifungal agent on amylose tris[(S)-α-methylbenzyl carbamate] based Chiralpak IH column. The effect of organic modifiers (methanol, ethanol and isopropanol), column temperature and back pr...
A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin). The impact of different stationary phases, co-solvents, column te...
A novel supercritical fluid chromatography (SFC) method is described to analyze four antihypertensive drug combinations containing a diuretic (hydrochlorothiazide, HCT), a calcium channel blocker (amlodipine, AML) and an angiotensin receptor blocker (olmesartan (OLM)/ telmisartan (TEL)/valsartan (VAL)/renin inhibitor (aliskiren, ALI) using a custom...
A facile supercritical fluid chromatography method is proposed to analyse 15 co‐formulated binary anti‐hypertensive drug combinations using a customized elution procedure. The effect of mobile phase composition, column back pressure and temperature was suitably optimized for adequate retention, analyte response and resolution. The chromatographic s...
Enantioseparation of three β-blockers, atenolol, metoprolol and propranolol is studied on amylose tris(3-chloro-5-methylphenylcarbamate) immobilized chiral stationary phase using supercritical fluid chromatography (SFC). The effect of organic modifiers (methanol, isopropanol and their mixture), column temperature and back pressure on chiral separat...
Tumor suppressor protein p53 is one of the most appealing targets for targeted anticancer therapy, due to its significant role in cancer prevention and abundant mutation in human cancers. A novel series of 4‐phenyl‐5‐(thiophen‐2‐yl)‐4H‐1,2,4‐triazole‐3‐thiols having ability to liberate p53 function by interrupting p53‐MDM2 interaction were successf...
Cellular tumor antigen p53 is significant for cancer prevention and its mutation is most documented genomic change in human cancers. Thus, restoration of p53 function by interruption of the p53-MDM2 interaction opens up a prospect for a nongenotoxic anticancer therapeutic strategy. A novel series of molecules comprising 1,2,4-triazole-3-thiol scaff...
Supercritical fluid chromatography envelops every chromatographic strategy where the versatile stage is supercritical under the states of investigation, with the solvating properties of the fluid measurably affecting the separation. Generally, for the examination of pharmaceuticals, HPLC or UHPLC is the procedure of decision for the partition and q...
Application of Molecular docking for selection of stationary phase for chiral separation of Molecules. Chromatographic trials and Molacular docking trials are compared and showed the effect of pH and Co-solvent in Supar Critical Fluid Chromatography.
Utilizing molecular hybridization approach, a progression of novel pyrazolylpyrimidine based N-thioamide derivatives of piperazine were identified in an effort to develop newer antibacterial and antitubercular agents against the cumulative bacterial resistance. Spectral analysis using Mass, ¹H NMR and ¹³C NMR spectral techniques have been studied i...
Routine qualitative and quantitative analyses, separations of complicated matrices, preparative scale separations or chiral separations are common and irreplaceable part of pharmaceutical, agrochemical and food productions. Nowadays, high performance liquid chromatography (HPLC) predominates as separation technique in both analytical and preparativ...
The details of Super critical fluid chromatography is explained with examples. Applications are also discussed on development of Anti hypertensive drugs.
The applications of super critical fluid chromatography was detailed and explained the possibility of novel method development on different trend of cinematographic separations.
Flower has enormous uses for several purposes, and by next day all that flowers are thrown in waste. The waste could be utilized as fertilizer in farming, but it is not fruitful because of contamination issues of waste after some time. Since this flower waste has many important ingredients which can be extracted from waste flowers to use as many ap...
The present study describes complexation of thymidine analogue antiretroviral drug, zidovudine with Fe3+, Co2+, Cu2+ and Zn2+ metal ions in methanol by conductometry and spectrophotometry. The stoichiometry of the resulting metal ion-zidovudine complex was 1:1 for all the metal ions, as evident from the plot of molar conductance vs. mole ratio of z...
We are using Water's PDA Detector and engineer prefer to measure energy with specific methanol and @ 230 nm,
Any specified reason to measure on this particular wavelength?
We are using Waters PSFC-200 with auto-cycle tank. In tank we are getting CO2 supply from Liq CO2 cylinders. During purification CO2 was provided with auto-cycle tank. After almost 7/8 hours we lost the Liq CO2 density in the system so we change the supply cylinder.
Interestingly, sometimes if we attach new cylinder with tank still we are not able to achieve the density.
We attached one condenser chiller at 8 degree C.
I know there is a big role of temperature and pressure for the conversation of CO2 in to its liquid foam. Anyone have suggestion to maintain the Liq foam of CO2?
I am using autodock tools for molecular docking. After docking i am finding binding energy. I want the information regarding bond angle and bond length. is it possible with autodock?
We are using CO2 pump having comparability of 200 ml/ min flow rate.
Usually we observe same amount of flow respect to RPM.
When system was shut down more than two hours, we facing excess RPM issues to start the system on routine flow.
80 ml flow set flow= 80 ml set flow = 78-82 RPM
After long shutdown time
80 ml flow set flow = 4 ml actual flow = 250+ RPM
After 30 mins of equilibration trials system is working proper, is their any precaution or simple way to deduct this down time?