Questions related to pH
I dissolve 2 mol/liter of Fecl3,6H2O and engineered biochar. finally, its pH value becomes negative. what is the factual background of this process? How much NaOH should I add to neutralize?
I'm studying heavy metal adsorption with some absorbents.
But At the adsorption isotherm experiment,
The pH decreased by increased concentration in the solution...
Is it normal Common phenomenon?
I couldn't find a paper that considers this phenomenon...
Murphy and Riley's colorimetric methods are suitable for alkaline samples. For sediment extracted with strong acids (pH of the supernatant is 0.8-5), what method and solution are suitable for colorimetry?
I'm designing a generic HILIC gradient to separate a complex mixture containing an unknown metabolite of interest for structure elucidation. The properties of the metabolite are unknown beyond its molecular weight and when it elutes on a C18 reverse-phase column (it is a polar molecule). I'm having difficulty finding online what a good buffer and pH is for a generic HILIC gradient, does anyone have any suggestions? I'll be using ACN and H2O as my solvents. My column is a Poroshell 120.
Murphy and Riley's colorimetric methods are suitable for alkaline samples. For sediment extracted with strong acids (pH of the supernatant is 0.8-5), what method and solution are suitable for colorimetry?
I synthesized Hydroxyapatite by wet chemical precipitation process with the help of a phosphate and calcium precursor and maintaining the pH of the solutions after mixing at different values using an alkali solution. I found that the percentage yield of hydroxyapatite varies with varying pH values while rest of the synthesis parameters were kept constant throught the synthesis process. What may be the exact reason for this variation in the yield of HA with varying pH? Thanks in advance.
Farmers of western Rajasthan face a lot of problems due to saline-alkali soils and poor quality irrigation water. Any instruments developed so far or any techniques may be suggested
I am utilizing different kind of clay in my experiment but its pH is biggest hurdle as working environment is acidic and when this clay is mixed with other material ,its not properly working i.e. its loosing its inherent properties . So I am looking for pH reducer (not acidic like H2SO4/ HCl). when I am utilizing acidic material my clay properties drops drastically.
I'm trying to make a solution for haptoglobin measurement using this product. My method gives me the following directions:
0.6g o-dianisidine, 13g sodium phosphate monobasic and 0.5g EDTA dissolved in 1l deionised water, pH adjusted to 4.1.
I know that o-dianisidine is only slightly soluble in water (60mg/l) but possibly more soluble under acidic conditions however it still did not totally dissolve when at pH 4.1
If I dissolved in alcohol, approx (50ml) does anyone know if the addition of the alcohol to my buffer will affect the assay result ?
Anyone have experience with this colorimetric assay for haptoglobin? solving the same amount in
I prepared a pea protein solution (pH≈7) and used the DSC to characterize the denaturation peaks. I run the DSC twice for the same sample. In the first run, I heated the sample from 25 to 130℃ and kept it at 130℃ for another 30 min. After cooling, I perform a second run. However, I found the DSC profiles of pea proteins didn't change, which is inconsistent with previous literature. What's the problem? Thanks!
Hi, Recently, we bought BCIP/NBT (B1911) of Sigma, before, we've used the same reactives of Roche and that reaction are mixed with Phosphatase Buffer - pH 9,5. Now, we don't know if B1911 is used with Phosphatase Buffer, we looking for the datasheet in webpage but the information is not sufficiently clear and comprehensive, Can you help me about the protocols for Western Blot?. Thanks
How can we say whether the solubility of Ar is more or less than CO2. To be precise how many times more or less is the solubility of Ar in Sea water than CO2?
Here are the details to the experiment
Innoculum : Thickened Sludge
Feed : Synthetic acetic acid and butyric acid
Reactors : CSTR reactor with working volume of 11L
pH at the start of my experiment : 7.4
I collect samples from the reactor everyday to check the pH of the system, and even though I'm feeding it synthetic organic acids around the pH of 3, the overall pH of the system steadily increased and now it's around 9.
My theory for the increase in pH is
1. The CO2 production caused production of bicarbonates
2. Some part of methanogenesis increases pH heavily.
If there's anyone who has had a similar experience please let me know.
I have PEGylated antibody in 6.5 pH phosphate buffer. I want to show change in molecular weight using SDS PAGE. While preparing the sample should I change the buffer of my sample to TRIS-HCl pH 8.8 or pH 6.8? My concern is if the pH 6.5 will affect the running process or not.
During Elution of protein (say an Antibody) from Protein A column using Elution buffer which is at low pH (3 to 4) and immediately adding Neutralization buffer to the collected fractions to prevent the negative effects due to this extreme low pH. I'ld like to know during this process will the protein remains in native form only (or) It will first unfold during low pH elution fallowed by re folding during neutralization ?
I am currently working on Lactobacillus salivarius for an eventual bacteriocin production, I don't adjust the pH of the supernatant when realizing the deep well diffusion agar test. To check wether the prior inhibition effect of the supernatant is due to pH or to other inhibitors in the supernatant, I prepared a control of MRS-Broth-HCl solution at a pH of 4 that I tested against the same bacteria. At the same time and same plate, I tested again the supernatant that I have also at pH 4 , and surprisingly I got an inhibition only for the MRS-HCl and nothing with the supernatant, even though it is by pH 4 . Do you have any suggestion that may help ? Thank you very much !
The role of conductivity concentration increases or decreases in the ANAMMOX WWTP in terms of nitrate, nitrite, ammonium and also pH. It affects more or less.
Can ignore the conductivity or it helps in understanding the behaviour of the SBR tank?
Your answer is valuable to me.
I am having an issue with measuring the pH of my media.
I use D-MEM/F12 media (without buffer) and try to adjust it to pH = 7.4.
Since I incubate it @ 37C / 5% CO2, I added 2.5 g/L of NaHCO3 (based on
However, the rapid change of pH due to air exposure prevents me from obtaining a reliable result. I did try to use 15ml open tubes for the incubation, which I tightly close before removing from the incubator to limit air exposure, but it does not seem to do the trick.
Any ideas/creative solutions?
I'm currently using HEK293T cells. Most spinfection protocols I have found did not state clear about if CO2 is needed during the spinfection in centrifuge after cells and virus are mixed. As 5% CO2, usually from the incubator , is necessary to maintain correct pH, would complete DMEM containing 5% CO2 be needed?
According to Kokubo's instuction for preparing SBF (Simulated Body Fluid) pH should be increased from 2.0 to 7.4 after adding Tris to the solution. I added all additives one by one, and the pH became 1.8-1.9 right before adding Tris. But then after adding Tris it didn't increased.
Does it mean the Tris composition is wrong? or Should I consider something especial in adding Tris?
(The Kukobo's paper has been attached.)
Thank you so much in advance for your replies.
Hi every one,
I am extracting enzymatic extract from plant (50 mM Sodium phosphate buffer (pH 7.8), 0.1 mM EDTA, 0.1 % (v/v) Triton; 1 mM PMSF). I mesure the protein content and enzymatic activity fom this extract.
So I want and I need to know if the enzymes extract can be conserved for further enzymatic activity measurement (SOD, CAT, APX, ...).
I really need your help, thank you very much
We would like to identify the % ratio of heavy metal ions species in water solution according to pH and concentration; for example: Cu2+, Cu(OH)+, Cu(OH)2.... Could you recommend a suitable software in this case? Thank you!
I want to apply a difunctional electrode in H-type two-electrode cell. I would want to know that if there is any way to use different pH electrolytes in the H-type two-electrode cell? Like 0.5 M H2SO4 on one side, 1M KOH on the other side? If there is possible, which ion exchange membrane should I choose?
And I am looking for an article which topic is about organic oxidation at the anode in 1M KOH and hydrogen generation at the cathode in 0.5M H2SO4. I appreciate that if you could tell me the name of similar articles.
I am stir washing (powdered) biochar aqueous solution. Washing is meant to increase the pH of the solution from 0.1 to 6. Each wash is carried out for one hour. Then 90% of water is drained and refilled with fresh deionized water and temp is maintained at 70C. If I run out of deionized water, using distilled water is equally good and will serve the purpose stated above?
I was doing DNA precipitation (after phenol-chloroform) with 2.5x volume 100% ethanol, 1/10 3M sodium acetate pH 5.2 at -80C freezer for 2h. After the incubation, the solution kind of half froze and turned into a glycerol-like consistency. I have a feeling this is not normal and the solution should be watery? What do people think?
I am trying to increase the pH of a powdered biochar aqueous solution up to 6 by stirring/washing with de-ionized water. The starting pH was 0.1. The initial weight of biochar powder was about 200 g and it was taken in 500 ml glass beaker and beaker was filled up with DI water. The temperature is kept at 70C and stirring is done for 01 hour, then I take off the beaker, let the powder settle down, drain the 90% of water from top, refill the beaker with DI water and repeat the stirring. I achieved a pH of 4.2 in about 35 washes but after that pH is not increasing and is staying at 4.2 even after 80 plus washes.
Any reasons or suggestions are requested for the stated problem please?
It is well known that increased atmospheric carbon dioxide has increased marine pH. Is there any evidence that increased atmospheric carbon dioxide has increased the pH of soils and/or caused any other land consequences due to changed pH.
We are trying to making nano ferrites like Zinc, Nickel, cobalt, copper, etc. via the hydrothermal method. In some cases, I am facing problems to maintain the pH of the solution, need to maintain the pH in a basic medium so all experts please put your opinion.
I have tried below this procedure but it won't work
so, I need new protocol
Procedure for estimation of CaCO3 in sediments
Preparation of reagents:
- Standard EDTA (salt) of 0.01M: Take 3.7984g of EDTA and dissolve in l liter of distilled water.
- ? Standard Ca+ (CO3) solution: Take approximately 3g of CaCO3 in a beaker and keep it in a desiccator for a few hours. Then weigh 0.25g and transfer it to a 150ml beaker. Add a few drops of HCL (½ to 1ml). Once the effervescence (escape of gas from an aqueous solution) stops, make it to 100ml with distilled water in a standard flask.
- NaOH solution/KOH solution of 8M: Dissolve 320g of NaOH in ~500ml of distilled water. Then make it up to 1 liter or dissolve 488g of KOH using distilled water and make up to 1 liter.
- Patton and Reeder's indicator (HHSNNH): mix 0.5g of the indicator with 50g of Potassium chloride (KCL) and using a mortar and pestle to power them as fine as possible (face powder).
- ? Hydroxylammonium (hydro)chloride.
- pH paper: use pH paper ranging from 0-14 (Whatman pH paper)
Procedure for calcium carbonate estimation:
- Take 0.1g of dried sample into a beaker, add 25ml of hot 1N HCL (36 ml of HCL in 1 liter) and wait for 10 min.
- Filter the solution and make the volume to 50ml with distilled water.
- Add 5ml of 8M KOH /NaOH and stir well and make sure that pH is ≥ 12, if less add up a few drops of NaOH /KCl.
- Then add 1ml triethanolamine and 30-50 mg of Patton and Reade’s indicator solution which turns the color of the solution to wine red.
- Titrate against EDTA till the blue color of the solution appears. Note down the burette reading which is equal to the amount of CaCO3(%).
CaCO3= volume of EDTA solution drawn down.
I wonder if it is possible to find natural soil carbonates (calcite, dolomite, etc.), not coming from liming, in soils naturally having a low pH (4-5.5).
Is it possible to find these mineral forms of C in acidic tropical soils?
I am asking because while measuring both total C and inorganic C (after acid dissolution) of tropical soil samples from Indonesia with an Elementar, I sometimes get a gap between the two measurements.
Sometimes the gap is positive (total C > organic C), and other times the gap is negative (organic C > total C !?). Generally, total C is equal to organic C, meaning most samples do not show these confusing 2-way gaps, and suggest the absence of inorganic forms of C.
In both cases, I wonder if discrepancies are just technical (noise), or if the gaps between samples are due to the natural variability of my samples, or in some cases, there could be some carbonates present in those soils (which have a relatively low pH of 4-5).
I have to perform WB on FFPE tissue. I've already done several attempts and the result is always the same. Can you help me understand why the bands are like this and not neat? Standard is fine (I didn't put it this time but it worked fine every time), so something went wrong before.
- It's dog breast cancer tissue
- Deparaffination was done with xylol and a series of alcohols (there was a series of steps with centrifuging and vortexing involved)
- Pellet weight was 45 mg
- I put 100 microliter of buffer in each tube
- It was all pretty homogenized
- I tried different buffers: (1) 200 mM tris-HCl, pH 7.5, 200 mM NaCl, 5% SDS, 100 mM sodium citrate; (2) 200 mM tris-HCl, pH 6.8, 20% glycerol, 2% SDS
- I incubated the samples in a Thermobloc with a shaker for 20 minutes at 99°C and for 2h at 80°C at 100 rpm
- The gel ran for 30 minutes at 200 V
- Running buffer was already done from Bio-Rad (Tris-glycine-SDS): it was diluted depending on the manufacturer's instructions
- The materials (including gel and running buffer) are pretty old, but they told me that they should work anyway and that the problem is probably another one
I'm a beginner in laboratory, so you can say everthing that comes to your mind, even if it's something obvious.
Thanks for your help
SCMs: Supplementary Cementitious Materials
CH: Portlandite (calcium hydroxide)
How to compute physical properties (thermodynamic parameters like free energy and kinetic parameters like rate) at different pH using computational software?
Suggestions of software/open source codes that can handle the problem is highly appreciated.
Solution A: 0.2 M NaH2PO4 solution Weigh 31.21 g of analytically pure NaH2PO4*2H2O and dilute to 1000 ml with distilled water.
Solution B: 0.2 M Na2HPO4 solution Weighed 71.64 g of analytically pure Na2HPO4*12H2O and dilute to 1000 ml with distilled water.
I'm relatively new to patch clamp, but I've been working extensively on optimizing our recording parameters for studying sEPSCs in adolescent rat CA1 pyramidal cells. My biggest hurdle has been consistent cell health in my slices. Some days, cells go to gigaohm almost instantly upon releasing positive pressure and applying minimal negative pressure. Other days, I cannot get a seal above 250 MOhm. I am currently using Jonathan Ting's NMDG and HEPES based solutions since I have had better luck with this method than traditional cold sucrose slicing. Osmolarity and pH are always properly adjusted (300-310 mOsm; pH 7.3-7.4), and solutions are well saturated with carbogen prior to slicing and recording. Each animal undergoes transcardial perfusion before slicing. I have tried slicing in both frontal and horizontal planes, and horizontal slices seem to produce the best cell health. However, I prefer the orientation of frontal slices when targeting this cell type since dorsal hippocampus has been used for many of our past experiments (field recordings, etc) and since the PC layer is so much more prominent. Lastly, I am using a Cs-gluconate based internal solution. At most, I can get 3 cells per day. Some days though, I cannot get a single acceptable recording. I guess I'm just looking for any tips from veterans of patching that may improve my throughput in these experiments. It is well known that patching older animals is sometimes a challenge, but surely I can improve what I'm doing in some way. I'm happy to provide any further details should the need arise. Thank you in advance for your suggestions!
What is the most appropriate system that simulates the physiological system (pH 7.4) for studying E/Z isomerization (inversion) of small organic molecules? How to prepare it?
I have prepared some nanoparticles encapsulating curcumin and I need to do a release test at pH 7.4 with these formulations to prove the sustained release of curcumin. However, as far as I know, curcumin is susceptible to quick degradation at this pH so this test seems unfeasible. I have found a lot of papers with release test of curcumin under sink condition at pH 7.4 but none of them mentioned the possible degradation of curcumin during the test. I don’t know if they intentionally or unintentionally ignore this problem. Does anyone have experience on this test or know some articles with reliable method for this? Thank you in advance.
I'm working on an industrial wastewater mainly composed by DMF and alcohols. I'm treating samples with hydrodynamic cavitation, hydrodynamic cavitation/H2O2 or hydrodynamic cavitation/O3 but at the end of each process the COD value is slightly higher than the wastewater one. I tried to remove excess of H2O2 by heating the samples at 90°C or adjusting pH to 10-11 and then heating at 45°C because of its interference, but also other samples have same problem Hannah Instrument COD kits are used to determine COD values.
What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?
The reason why I don’t want to use NaOH to adjust its pH value is because the gold solution (HAuCl4) starts to precipitate when adjusted to pH>7.
Looking forward to comments and suggestions to the following:
It is quite common to use chlorine-based (sodium hypochlorite) Open Plant Cleaners (OPC) foam cleaners in slaughter houses. Using such cleaners can however lead to high levels of AOX in waste waters.
To adjust the final pH of the waste water carbon dioxide (CO2) is used instead of using standard acids, e.g. sulfuric or hydrochloric acid.
The CO2 is injected via a nozzle. The question is whether there is such a significant drop in the pH value at this point and whether this promotes the formation of AOX due to the higher reactivity.
Are there any extra ways we can reduce the AOX levels, considering high AOX levels cannot not be avoided when using hypochlorite-based cleaners?
Looking to response!
Part of my thesis is to measure the pH of aqueous extract of chaga, aw and dry matter of powder chaga, TCP (g GAE / kg) and antioxidant capacity (DPPH in %) in aqueous and methanol extract. I need to compare my values with others, but I can't find relevant sources. Please help.
We made aqueous extracts of chaga with a water temperature of 20, 50 and 100 ° C. The extraction time was 5 minutes.
Any results will help me.
For a research project, I want to measure enzyme activity during the process. Is it possible to measure this with a pH meter? In some results I already saw a pH change, however for me it's not clear what the enzymes do on the pH.
I have investigated that synthesis is done in a basic medium to obtain three-dimensional particles; but I have read that in some media they say that the particle size increases if the pH increases, in other places the particle size decreases if the pH increases. Could someone help me or explain this?
I have observed this in case of both Goat serum albumin and Bovine serum albumin addition to HBSS where the pH of the solution reduced from 7.4-7.6 to 7.2-7.3. Now, looking for the reason.
why in research papers people are always expressing the acidity of crude oil by total acid number and total base number instead of measuring the pH of the crude oil ?
What will be the products when vitamin C reacts with hydroxyl free-radicals (OH.) at various pH buffers (pH 3, 4, 5, 6, 7, 8, 9, 10, 11, 13). The buffers of pH 3-7 contains citric acid (C6H8O7) and disodium hydrogen phosphate (Na2HPHO4), pH 8 contains disodium hydrogen phosphate (Na2HPHO4) and sodium dihydrogen phosphate (NaH2PHO4), pH 9.8 and 11 contains sodium hydroxide (NaOH) and sodium bicarbonate (NaHCO3), and pH 13 contains potassium chloride (KCl) and sodium hydroxide (NaOH).
Thanks in advance for the expert opinions.
How to determine precipitation during adsorption of heavy metal ions
I am purifying a recombinant protein in E.coli, with an expected molecular weight of 17 kDa and a PI of 4.6. When I put my protein sample on gel, I see a band appearing around 30kDa, so quite big but this is not the mayor issue here (although input is always welcome): This 30 kDa band is visible when I run my SDS-page with Mini-PROTEAN® Tris/Tricine gel (4-20%). When i run the same sample on a Criterion XT gel with Bis-Tris-HcL MOPS buffer system, i see this band arround 24 kDa. Any idea why this could be? I also have another protein with similar PI (and expected size of 40kDa) that shows a band around 50kDa in the Tris-Glycine but around 40 in the Bis-Tris MOPS.
I have been struggling trying to create a standard curve with cisplatin. I have been using the method attached in the article below.
Here is the method I have been following...
1. 10 mg cisplatin HCl was dissolved in 5 mM phosphate buffer pH=6.8 in a 100 mL standard volumetric flask and diluted using phosphate buffer to make 100 ug/ml cisplatin stock solution.
2. 10 ug/ml cisplatin solution was made by diluting 10 mL of stock solution in 100 mL volumetric flask using phosphate buffer.
3. from 10 ug/ml cisplatin solution, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 mL aliquots were withdrawn and placed into 10 mL standard volumetric flask.
4. 1 mL of 1.4 mg/ml OPDA solution (made in DMF pH=6.4 adjusted with 0.1 N HCl) was added into each solution.
5. 2 mL of phosphate buffer was added to each solution
6. the solutions were heated on a hot plate at 100 C for 10 minutes
7. solutions were cooled to room temperature
8. solutions were diluted with DMF
9. absorbance was measured at 706 nm using DMF as blank (750 baseline correction).
I am really not sure what I am doing wrong. I believe I am following the protocol correctly but when I measure the absorbances they are all reading low and relatively the same value. What might I be doing wrong?
My possible guesses....
1. cisplatin stock solution is not fully dissolved and should be heated?
2. the standards should be heated longer than 10 mins.
3. using a hot plate is incorrect?
4. temperature is actually lower than expected because I am heating all the standards together at one time?
thank you in advance for your help and suggestions
We have a Hepes that is 1 M. We require 5-10 mM Hepes for balance pH in cell culture medium. What should we be added amount of water to prepare for the level of 5-10 mM Hepes?
I'm preparing enzyme-responsive polymeric nanosystems and according to the manufacturer's information, the enzyme is in
-» "50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.";
-»"≥10 units/mg protein" and
-» unit definition "One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of compound x per min at 40°C, using 100 mM Na+/K+ pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer."
The flask has 50 micrograms of enzyme.
Concentration: 0.451 mg/mL.
I wanted to prepare 0.5 UN/ mL solution.
Thanks very much
I am looking for a buffer system maintaining a pH range of 6.1 – 6.7 which does not interact with the metabolism of yeast cells included in the reaction solution (positive and negative interaction).
I'm preparing modified simulated body fluid for bioactivity tests,, but the resulting solution has always pH of 6.11 to 6.17! which is too low obviously!
and when I add 1 M NaOH to adjust the pH as mentioned in Ayako Oyane paper (2003), the solution start to have very pale yellow color with precipitations in it, although before adding NaOH its like a water clear without any precipitations or color
How can I adjust the pH without changing the color and clarity of the liquid???
I need to determine the volume required of sulfuric acid to be added to my process to decrease the pH of my e. coli fermentation solution from 7 to 5. The initial volume of solution is 12,000L and pH of sulfuric acid used is 0.3.
- Initial pH = 7
- Initial volume = 12,000L
- Sulfuric acid pH = 0.3
- Volume of Sulfuric Acid = ?
- Desired pH = 5
- Final volume = 12,000 + V(H2SO4)
Hi I am trying to create a bioassay that relies on magnetite nanoparticle (100nm) having a shell material that has NO peroxidase activity. I am trying to coat the particle so that the outside environment does not contact the magnetite core. The shell material must be stable at pH 4 and not allow Fe ions to leak out. Therefore, silica is not an option. Is there any material that fits this purpose?
i a question. I have the need to measure soluble collagen from colon mouse tissue but i do not have freezing colon samples but only the protein extract for wester blot analysis (- Hepes pH 7,9 -EDTA pH 8,0 -KCl -Nonidet; DTT, PMSF, Aprotinin , Leupeptin, Na3VO4). Do you think that I can quantified collagen in this protein extracxt?
thank you all
The pH of Fe(II)(Cysteine)2 was 7 in my experiment, and after 8% O2 injection, pH was increased to 8.3
Could you teach me why this phenomenon is occured?
How can I calculate lime requirement for increasing soil pH without Lab determine? or which methods are faster and easier than woodruff buffer solution?
I ordered Type 1 collagen from sigma (C9879) and followed the instructions to dissolve in 50mM TES buffer with .36mM CaCl2. I am currently trying 2.5mg/ml and it is hardly going into solution.
I am worried about making the solution too dilute as the pH can affect the overall pH of my gels once solubilized.
Does anyone have experience making collagen zymography gels? If so any input would be greatly appreciated!
Recently I've been looking for a strict guidelines considering the stability of chromatographic mobile phases. Found none, only some hints, good practice clues or SOP's from different labs all based on "scientific judgment". It is great for RnD, but not enough for GMP/ISO.
I bet many people here already dig this topic inside out. Can anyone direct me towards clear guidelines or possibly share the methods of testing the stability of the different mobile phases (besides pH checking) that she/he uses?
Any "hard facts" would be greatly appreciated especially for those working with routine and GMP analyses, and before audit :)
pH can influence the efficiency for pesticides. We also know that hardness of the water can influence the availability of nutrients in fertigation systems.
How do both of these parameters influence the effect on foliar application of fertilizers.
There is one interesting article on effect of pH on cotton for foliar.
Do people know more articles or have experience with the effect on efficiency or phytotoxicity?
I have PEG conjugated antibody in pH 10 borate buffer. I have to run SDS PAGE to determine MW. In this case prior running the gel do I need to do buffer change of the sample? Like borate buffer to PBS?
I am trying to understand the cause of cell lysis (specifically red cells) in ultra-low pH ranges (between 1 and 2) but I am struggling to find papers/research below pH 3. Is there an obvious reason for this? Many thanks, Niamh.
I am focusing on researches dealing with evaluation of the soil biodiversity associated with tea orchards, in which the pH of soil is very low ranging between 3 and 4. Regarding this, any opinions can give us suggestions in improving the quality of these acid soils of tea gardens by applying biochar in order not to lose its biodiversity.
I have to perform Western Blot on FFPE tissue and the first part is the extraction.
First I did deparaffination, then I added the extraction buffer (20 mM Tris-HCl pH 8, 2% SDS) and I incubated for 20 minutes at 99°C and for 2h at 80°C.
This is the result with and without pellet. Can I understand by eye if the proteins are in here? Is there some clue that makes me understand that the lysis didn't occur correctly? Or do I have to wait until staining with ponceau S?
Someone that did something similar could tell me if this is the right apperance?
Thanks for your help
working my very first time with hexachloro-cyclotriphosphazene, I was very careful because hexachloro-cyclotriphosphazene is known to react with water very vigorously.
However: Nothing happend in my experiment. Not in water, and not @ high or low pH or @ elevated temperatures.
Can anybody explain this?
Thank you in advance!
It's a buffer for protein extraction from FFPE tissue, but it's not actually important for the answer. I just want to understand what to do exactly
I want to prepare 100 mL of this buffer:
20 mM Tris-HCl (pH 8)
I took 0,24 g of Tris and put it into the beaker, I added 0,1 mL of HCl, added 2 g of SDS and filled with dH₂O until I reached 100 mL
Now, my doubts are:
- Should I at first prepare 20 mM Tris-HCl and only after that add it to the 2 g of SDS until I reach the desired volume?
- Some people told me that actually "2% SDS" means that I have to first prepare a solution with 2% SDS and distilled water, and only after add it to a 20 mM Tris-HCl solution. If it's so, how can I understand how much volume of Tris-HCl and 2% SDS should I put in my solution to reach 100 mL?
Thanks for your help
Current I am using calcium hydroxide but at higher pH it make gel type material
I'm reading a research paper that stated the pH value of the decreased due to the production of carbonic acid and other organic acids. Therefore, I want to know what types of carbonic acid and organic were produced that caused the pH to drop after the fermentation.
For protein collection I used Ripa buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS, ) and put into ice immediately after collection. After 30 min incubated, centrifuge 12,000 rpm and collect supernatant and quantify protein. 15 ul to 20 ul protein loaded where protein concentration 40 ug/mL. I used cell and usually culture in 10 cm petri dish plate.
Dear all, I am studying microbiology for mater degree course.
So now I am performing MTT assay, and have some questions.
I use HT-29 cell with 5 x 10^4 seeding level and have 3 samples with 4 concentration for treatment group, use DW for control. I would call samples A, B, C for understanding.
I treated all samples for 18 h, and treated MTT solution for 2 h, using DMSO as final solvent and reading O.D at 540nm wavelength. MTT assay performed 4 times.
sample A and C showed 80~120% cell viability in every concentration.
BUT only B showed overflow in almost every well or 600~800% cell viability at lowest concentration in all test.
I checked pH of sample solutions, B and C have pH level between 4.8-5.0, A was about pH 6.3
Does anybody have similar experience? Please give me some advice.
During the synthesis of nanoparticles, many at times a particular pH value is optimum for that process.
I am confused as to whether the pH needs to be adjusted during the reaction while mixing the precursors, or after mixing the precursors (followed by continued stirring for a certain period of time).
Does it depend on the reaction or is there a preferred way?
I am asking this as I have found both of the above mentioned approaches in papers.
For example for the synthesis of covellite copper sulide (CuS) nanoparticles:
According to some research, the pH of Tris-HCl depends on temperature. I have also checked that if I prepare Tris-HCl at room temperature, then put them at 4℃. As a result, the pH improves from 7.5 to 8.1. And I hope to make the protein stay at a low temperature which can ensure the structure and biological activity. So, maybe storing the stock of Tris-HCl at 4℃ is better. But, If I want to use the protein to try growing crystals, usually, the crystal plate was put at room temperature for two weeks first. I just worry about if the changing of buffer pH would have a bad effect on the crystals. Except putting the crystal plate at a low temperature first, is there any good method to solve this problem?
I am doing research in land suitability evaluation for Ginger production and I need to assign the weights for the analysis factors. I need experts decision about the importance of the factors affecting ginger growth. Can you help order the factors from the most important to the least important?
For example, if you think that pH is more important than OM for Ginger and more affecting the wheat growth you write in your answer like this pH > OM. Or Using satty scale (0-9) to give relative importance of each factors.
The factors or the parameters are 9:
pH, Slope, Soil depth, texture , Organic matter, N, P, K.
Please order using “>” simple. or satty scale of preference
I have established linearity for my sample in HPLC, still the LOD is not to the required mark(at the present the method can detect 200ng/ml). But I want the method to be sensitive enough to measure 25-50ng/ml. I used a method from the paper but without adjusting pH. Will pH adjustment increase the sensitivity?
What are the general ways to increase the LOD for the method you have established?
41.0 mL of 0.0150 M hydrochloric acid, 130 mL of 0.0650 M hydroiodic acid and 55 mL of 0.00680 M sulfuric acid are pipetted into a 250 mL volumetric flask, which is then filled up to the mark with distilled water. What is the pH of the final solution?