Questions related to Methods
has anyone used Zeba spin desalting columns?
Can they be reused?
They are quite expensive and it would be great if they can be reused...
Also, manufacturer (Thermo Scientific) hasn't provided information whether they are polypropylene/polyethilene/dextran/sephadex or whatsoever-based columns...
I am planning a citizen science project where participants will be asked to log the occurrence and magnitude of gastrointestinal events throughout the day. Is there an app that would be well suited for this (for both iOS and Android)? Ideally one that requires only the press of a button to log an event to keep it as simple as possible.
Thank you for any answers
Results from qualitative studies cannot be generalized. Therefore, is it possible to propose a social practice model based on the findings that cannot be generalized? I see some published articles and thesis that present models as a result of qualitative research. I will be glad if you help, thanks in advance :))
i need your help..
im working now with my thesis..
i need some supplementary articles...unfortunately, i cant access here in our country articles from science direct....if you dont mind, kindly download this article for me...
Could anybody recommend a good method to isolate total RNA from human blood plasma?
We need to analyze some important patient samples. But we do not have whole blood - the technician only froze blood plasma! So I'm asking out of desperation.
We need to extract total RNA (we are particularly interested in mRNA). We have between 1 ml and 1.5 ml of plasma, according to the sample.
I am presuming that some cells might remain in the plasma after centrifugation (3500 rpm, 10 minutes) and its separation from the whole blood sample. Does anybody know approx. how many cells might be contaminating the plasma and what yield could be expected? Has anybody successfully extracted RNA this way before?
In your opinion, what is the difference between a good teacher and a great teacher?
and what are the main teaching styles and how they Impact Students?
I am looking for help on how to formulate a phrase.
I have excluded patients with entirely missing data, but have included cases with only incomplete documentation. How do I include this in my paper and express that therefore statistics and reported results do not align with the total number of included cases?
And where would you advise I put this information? Under the Methods section? Discuss it under limitations?
Thank you, I appreciate your help!
Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.
I am organizing some materials for the MSc and PhD students and want to categorize the references in some dimensions so the students can choose by their own.
I am specifically interested in works of art for which ratings on multiple dimensions are readily available--for instance, ratings of beauty or of liking that were acquired from a sample of volunteers. Features of interest also include the actual features of the artworks themselves, like brightness, complexity, etc. I have been pointed towards several options:
- Prediction of beauty and liking ratings for abstract and representational paintings using subjective and objective measures (https://osf.io/2sy4f/)
- JenAesthetics (https://www.inf-cv.uni-jena.de/jenaesthetics.html)
- The Strohminger Grotesque Art Database (https://ninastrohminger.com/grotesque)
I wondered whether there might be others I am missing? Thanks, in advance, for any help anyone is able to provide!
I plan on doing cell culture on Thermanox cover slips so that I can take TEM images of my monolayer cultures. I've seen that I can place the cover slips into well plates for culturing purposes, but I don't know how to go from "I have my cells in suspension, ready to be seeded" to "the cells are now seeded on the cover slip".
Do I have to be very precise with where I pipet the cell suspension? Do I just fill up the entire well with the cell suspension? Any insight would be very helpful!
As all of us are familiar with the different journal ranking systems and requirement conditions, in many cases we meet different kind of fees, charges for publishing our researches. Usually only the submitting and the previewing cost US$ 50-250, which is non-refundable and the paper may be rejected by the editors without being sent for review. Others introduce fees for the publishing US$ 500 -1000 (extra fees for colour graphs, maps etc. or for appeals against a Chief Editor's decision). For good English, they offer some links for the grammar review: US$ 100-200. Besides all of this, they employ embargo for 12-36 months, and ask US$ 600-2500 for open access. I think these fees sometimes unreasonable, so it is hard not to find the business factor behind them.
As it says in the title really. From what I can find online, a lot of literature focus on just OM proteins/vesicles rather than whole out membrane preps. So if anyone has some papers they wouldn't mind sharing, or know if there's a specific search phrase I should be using, I'd really appreciate a comment :D
Many Thanks in advance,
I am conducting a Large N fsQCA, and I need a basic bibliography on this topic. Especially on scope conditions, necessary tests, and its relation to the deterministic ontology of the method. Would someone indicates it for me, please? Thanks!
When looking into thematic analysis, you always find a notion of it as a method rather than methodology. Therefore, I was wondering, if TA can ever be considered as methodology? If so, under what conditions, and what philosophical stances does it represent?
If it's always just a method, is there any particular methodology that links with it?
I hope this makes sense. Thank you for your replies.
I have been trying to estimate some biochemical parameters in urinary bladder but homogenization is problem with me as indicated by very low amount of protein in homogenate.
I am working on an intervention study with 3 different groups of students. One group represents the intervention group and consists of a seminar with a practical phase and computer science content. Another group has only a theory seminar with computer science content and the last group has a seminar with other content. The last group is used to check how stable the constructs are. The 3 measurement points are equally spaced as pre-inter-post-tests in a quasi-experimental setting. Latent growth curve modelling doesn´t fit! Is there a method that uses the strength of the 3 measurement time points with a small sample size?
I am going to use this method to load protein hydrolysates into milk phospholipid nanoliposomes. However, I couldn't find a lot of information about this method. I'd appreciate it if someone can provide me some more information on this specific topic.
A few days ago a colleague of mine made me think about the impact the COVID19 crisis will have on cohort studies. Especially those focused on causes of mortality and the elderly will be deeply impacted by the number of deaths due to the pandemic.
Is this something manageable?
How big is this matter in your mind?
How can this be handled?
I need di- and tri-peptides for my intended work. I was going through internet and came across three different methods..1) by Solid state peptide synthesis (SSPS) 2) Liquid Phase Peptide Synthesis and 3) by Bacterial expression....However for first two..we dont have lab facility...so I wanted to know whether Bacterial expression could be carried out for synthesizing small peptides? Which one would be better?
I have a data which consists of an excess of zero counts. The independent variables are number of tree, diameter at breast height and basal area, and the dependent variable (predictors) is number of recruits (with many zero counts).
So, I want to use Zero-inflated negative binomial model and Hurdle negative binomial model to analyze. My problem is I do not know the code of these models in R package.
I am in need of a free on-line software for performing chemometric analysis and chromatographic alignment of retention time shifts. any suggestions!!
Also i have another inquiry about how the numerical data matrix of HPLC-MS/MS can be arranged? I mean how the data of time (min)/relative intensity (%) for MS, TIC monitored chromatograms can be composed in an excel spreadsheet file to be imported into the software?
Thanks in advance
Do kits such as the NEBNext microbiome DNA enrichment kits really work for the enrichment of (all) viral DNA and how species specific is the host DNA removal (I'm working with bovine blood samples)? Any practical experience?
I'm trying to find a home for an instructional manuscript on how to use NVivo to perform qualitative methods (abstract below). I've tried three different journals (Current Psychology, Psychological Methods, Qualitative Health Research) with no luck (they say its good content, but not a good fit). Any suggestions?
From 1995-2016, there has been a 15-fold increase in qualitative scholarship in the social sciences but the rigor and quality of published work has ranged widely. Little scholarship provides concrete, pragmatic explanation of (and direction regarding) execution of systematic, high-rigor qualitative analysis. The present article guides the developing qualitative researcher through technical and procedural aspects of analyzing qualitative data with specific attention to reliability and rigor. Guidance addressing transcription, importing data, forming coding pairs, performing initial/open coding (examples of three types), determining core themes, systematic team-based coding, maintaining a data audit trail, creating a Numeric Content Analysis (NCA) table, and preparing work for publication is provided. Material includes several tables and figures that offer practical demonstrations of how to use Nvivo in data analysis. Transcription tips and outsourcing benefits and cautions are also offered. Altogether, the present article provides qualitative researchers practical guidance for executing multiple stages of qualitative analysis.
I want to quantify the amount of D2O and HDO present in water below 1% level.
1) For now, I only need to know the amount of deuterium present in water.
2) But, it would be better if I can analyze the ratio of D2O, HDO and H2O .
Can you recommend an analysis method? Simple method is better.
If i want to study the experiences of ( students, teachers and administrators ) related to a new course by using a questionnaire with students and teachers and interviewing the volunteer teachers and the administrators.
So, I will use a quantitative design with students
a Quantitative and qualitative design with teachers ( I will interview just who are write his/her contact information on the survey tool) and
a Qualitative design with administrators.
Can I describe this method as a concurrent design?
we are working on isolating bacterial strains that produce secreted cholesterol oxidase using a minimum medium with cholesterol as the sole carbon source. I noticed that the cholesterol forms an emulsion and obviously doesn't like to dissolve well. So the bugs might see very little cholesterol in solution. This problem is not mentioned in the paper we follow.
The questions are: a) does that matter? and if so b) What do I do to get cholesterol dissolved? What would be a good detergent to use without killing the bugs?
" Pitfall trapping is the standard method for collecting ground-dwelling arthropods and soil fauna in studies of ecological and agricultural entomology " ( Ruiz-Lupión et al. 2019).
In my current research assistant position I am working on analysis of macro-fauna in forests. We use pitfall traps to assess the abundance of macro-fauna in a given area. I'm curious to learn more about other methods used for this sort of analysis.
- What methods for pitfall trapping have you used, if any?
- What were the advantages/disadvantages and what would you have changed about the method you used.
Our methods are as follows:
- Briefly, we plant a plastic cup in the ground with a cover on top (to make sure mammals or larger animals do not enter the trap but only macrofauna can enter)
- we leave the cup for several weeks
- The macrofauna fall into the cup and are preserved by antifreeze, which are then taken into lab for identification and abundance counts
- By measuring the area of the cup's top, and how many bugs have fell into said area, we can then gain a better understanding of the abundance of macrofauna in the area
In a study reviewing pitfall traps, Ruiz-Lupión et al. (2019) states the factors which should be considered by ecologists using pitfall traps. They state, "the capture rate of arthropods in pitfall traps is proportional to their activity, and the number of individuals that each trap catches may or may not reflect their true abundance, and instead just their activity. Thus, the rate of capture is proportional to the joint effects of abundance and activity, something that has very often been overlooked by ecologists for a long time... [Nonetheless,] activity estimates from pitfall trap catches can still be biased because of multiple factors such as the surrounding habitat structure or the environmental conditions such as temperature and water availability. Additional factors could be the vertical distribution of the soil and leaf litter layers, as well as the attraction or repulsion of preservative fluids, detergents, or baits, the effects of which vary according to the taxon, sex, season, and environment. Specifically, if a trap retains excessive amounts of water, it could act as an attractor for the fauna, especially during drought periods, therefore biasing the estimates of activity. "
Dolores Ruiz-Lupión (2019). New Litter Trap Devices Outperform Pitfall Traps for Studying Arthropod Activity. Insects 2019, 10(5), 147; https://doi.org/10.3390/insects10050147
I want to calculate Potential evapo-transpiration with less data requirement so which method can be used for this which will give accurate and appropriate results.
Hope everyone is doing alright during these turbulent and uncertain times.
I am working on my masters dissertation (MSc International Management) and would like to answer the research question of "how adaptable are frugal health care systems in developed markets?" (such as U.S.). I am really stuck as I cannot get hold of any experts to interview and data/literature is limited, as I have found so far. Does anyone have an idea of how I could tackle the methodology or has any tips on where to get data from in this area.
P.S. : Would not mind changing the angle on research question in order to make finding data/literature easier.
Many thanks in advance, feel free to contact me, if you have further questions.
How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?
Hi everybody. I am facing an issue about reproduction data analysis in chronic toxicity test with C.dubia. US EPA Method 1002.0 ( https://www.epa.gov/sites/production/files/2015-08/documents/short-term-chronic-freshwater-wet-manual_2002.pdf) instructions tell:
" The response used in the statistical analysis is the number of young produced per adult female, which is determined by taking the total number of young produced until either the time of death of the adult or the end of the experiment, whichever comes first. An animal that dies before producing young, if it has not been identified as a male, would be included in the analysis with zero entered as the number of young produced "
Including dead animals in the count gives me back too high standard deviation values which don't fit well in my analysis. I would like to know if alternatives are available: feel free to link me to other papers, works, methods etc.. which could help. Thanks a lot to anyone will spend time to help me.
I graduated with a Neuroscience and Psychology BSc in 2016, and my final year project was a bench-lab in vitro study of omega-3 oils applied to fluorescently-stained cortical neurons. I realised that although I had a passion for neuroscience, my first foray into applied research was not promising; I hated the tedium of the process.
I then did an MSc in Mental Health Sciences, with my thesis originally using an EEG/MEG dataset on psychosis patients. Again, I loathed trying to learn (the tedium of) Matlab and learnt instead that I'm fundamentally not a coder.
I changed to studying the mid-term after-effects of a psychedelic drug using a battery of questionnaires. I loved this psychometric approach, and it was easy enough running simple statistical analyses. However this was firmly psychological, not neuroscience.
Now I'm doing a PhD largely employing thematic and other qualitative analyses of altered states, which I thoroughly enjoy. But again, I'm still attempting to retain some know-how in neuroscientific methods. I'm doing some basic secondary EEG analyses on the aperiodic signal - though not loving even the rudimentary coding necessary.
My question is what are some other neuroscience techniques available out there which I may be more suited to? Can one even be a 'neuroscientist' without having to use a wet-lab - or matlab?
Perhaps tDC or tMS? Could someone maybe elaborate on the types of questions that can be answered with these; or the analyses that are run after them?
Other than f/MRI, which I assume always require programming - do other neuroimaging modes e.g. PET/SPECT also require this?
I'd also be very interested to hear people's thoughts on/experience with working to incorporate as much understanding of neuroscientific findings in strictly psychology studies - While writing psychology papers, referring strongly to the neuro literature to inform and discuss the study's aims and findings?
Thank you very much,
I'd be really appreciative of any insight :)
I am doing a plaque assay with HeLa cells and infecting them with poliovirus. I dont get plaques?
Method i use:
1. I take a 80-90% confluent plate of HeLa cells. I do the assay in the 6 well plates from Nunc.
2. Dilute the virus in serum free DMEM-10-2 through 10-11.
3. Wash the plates with 1X serum free DMEM.
4. I infect the cells with virus 300 ul for 30-50 minutes at RT/rocking.
5. Overlay with 5ml(2XRPMI without phenol red) + 2.5 ml 1.5% Agarose +2.5 ml 1.5% Agar.
6. keep in the 37 degrees incubator, 5% CO2 for 48 hours
7. Fix with 10%(v/v)formaldehyde in 1XPBS for 30 minutes.
8. Stain-0.5%(w/v) crystal violet in 10% EtOH for 5 minutes. and wash with water.
We want to do a study that test if the placement of your mobile phone effects your memory. If it being next to you, decreases your memory by disturbing your attention. So the phone will either be next to you on the table, in your pocket/bag or in another room.
Is it necessary to have multiple complex tasks, such as the operation span test and another one or is that one enough?
As we are quite short on time, studying long term memory is not possible.
Thank you for your answers.
I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual elements:
- Which are some suitable methods that can be applied?
- Should both areas of studies be represented in a balanced way?
- Any tip that you would have loved to hear before developing your own interdisciplinary project
I am interested in relationships between photovoice or auto-photography as research methods and social-spatial difference, either as captured in the photographs, or as embodied or lived by the participants. I would particularly appreciate suggestions of literature from the past 10 years.
Recommendations of reading on participant-photography and social-spatial difference would also be relevant in this case.
I am currently developing a project where the primary mode of inquiry is rooted in practice based methods and techniques. The project is focused on a social dance form. I have read several books/articles in which practiced based methodologies are used, however, I am wondering if there are methods/techniques out there that they better satisfy my project. What practice-based methods/techniques may be beneficial for examining social dance styles?
There are several methods for calculating Mixing Height but requires upper air data. Is there any source for finding mixing height using surface met. data. Kindly help me to find them.
I am going to update my methods of delivering lectures at university. Any publication link or comments on this regard will be highly appreciated!
Thank you in advance!
Dr. Vardan Atoyan
I need your professional opinion for my ongoing research. Any input, support, publication links or comments will be highly appreciated!
Thank you in advance!
Dr. Vardan Atoyan
Has anyone ever had the issue when they are doing Loss on ignition analysis in a muffler oven at 1000C? Previously I worked with a newer muffler oven and had no issues weighing and baking soils at 1000C. I recently started using an different muffler oven which is a little older, but is properly functioning. I baked my soils and crucibles at 550C and it was fine. I then turned it up to 1000C and baked it at 1000C for 6 hours and turned it down to 105C to take the crucibles out after cooling. However, after I took the crucibles out all of them seemed to be changing colors from neutral ceramic white to green from the bottom up. Also it looks like there are small crystals or precipitate forming on the crucibles, more densely from the bottom up. Attached are a couple of pictures. Does anyone have any experience with this or have any suggestions on how to get to the bottom of this or fix it?
Edit: I just want to note I tried to clean these crucibles with an HCl acid bath, and the greenness did not go away and the precipitate crystals seems to arise once it is fully dry again.
Our lab looks at tumor growth in the bone microenvironment, and in doing so, I section mouse tibia on a fairly regular basis. These tibia have been manually cleaned, fixed in z-fix for a total of 48h (24h at RT, change z-fix, 24h at 4 degrees), then decalcified (10% EDTA, pH 6.5) for a total of 2 weeks at 4 degrees (changed after 1 week). They were then rinsed well with deionized water, put into 70% ethanol, and sent to Histoserv for paraffin embedding and initial sectioning for H&E sections. I then section these blocks for addition staining, and most of the time, everything is okay. However, I come across some blocks where, for some reason, part of the bone will chip out during the sectioning (as the blade goes through the block, you can actually see the chip of bone come out). I have tried to re-paraffin these blocks by softening them, placing the chip back in place, putting a small layer of paraffin on top using a spatula, then re-molding the block. Sometimes this works, sometimes this doesn't. Does anyone have any suggestions for what to do when the bone chips out of the block? Thank you!
I plan to do an anthelminthic assay using Shorea leaves for my BS thesis and I consulted vets and nurses (unfortunately, we don't have a parasitology department or even a dedicated parasitology professor in our school) and have obtained a wide and contradicting opinions on the matter. I want to get results as close as possible to reality and so I plan to use A. suum as opposed to using C. elegans or P. posthuma. A vet I asked suggested I do an in vivo assay using lab rats as host and T. muris as model organism or A. galli on chicken host. Additionally, they said performing in vitro assay will invalidate my results as the conditions in an in vitro set up may be too artificial and may not reflect what is happening inside the body. One of the nurses I asked suggested I skip using model organisms altogether and use in vitro assay using A. lumbricoides or an in vivo assay of the same organism but on a guinea pig host.
I want to use a surface again, that has already been mounted with prolong antifade mountant and am looking for a removal method for this.
Any help is much appreciated
I have two methods I want to compare, however I have different sample sizes in each method. One has 12 samples and the other 25. The idea is to see if collecting more samples gives better results.
I was thinking a Bland Altman but don't you need the same sample sizes? Do I just do a normal unpaired t-test for this?
I want to detect 50-bp segment of the recA gene Bordetella avium. Seqence of probe and primers: 5-CATCGCGCTGGGTG-3 NED fluorescent reporter on the 5' end and nonfluorescent black hole quencher at the 3' end. Primer forward CGGTTCGCTGGGCTTGG and rewers CACGCGGCAGCCCGC. Can I change probe dyes to FAM on the 5' end and on BHQ-1 on the 3' end without losing specificity of reaction? I will be thankful for any help
I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other protein at around 40kDa (for b actin) I have no issues.
I have read that for larger proteins people either run overnight at 4 degrees at 20V or for 3 hours at 70V.
Ideally I would like to run in the shortest time possible! One of the reasons why is the power pack i use is pretty old and I don't know how well it would withstand the cold room (rusting)!
But my main worry is losing the smaller proteins if I up the voltage for a prolonged time.
I am using pre-cast tris-glycine 4-20% and the transfer buffer is composed of 10% methanol, and tris-glycine buffer (x2) for a wet transfer.
I was wondering what protocols work for you or if you have any suggestions on what I should try first?
I am currently comparing two groups (control/exp) using SEM. Each latent construct (5 in total) has at least 8 if not 20 individual indicators, as such I am using appropriate parceling techniques in each. The first step requires I conduct a CFA for each construct of interest - to do so, I must have at least four indicators. In the full SEM, as there are 5 latent constructs in total, to improve overall fit, I wanted to reduce the number of parameters to be estimated to be two indicators for reach latent, rather than three. My question is, is it permissible to run a CFA with three indicators for each individual latent, but in the final SEM - only two indicators (using an appropriate parceling technique - rather than just dropping items).
I am looking for any insight or proven practice regarding the usage of email for research. Is there a program/website/process to anonymize both a researcher and participants' email addresses but still allow them to communicate?
Craigslist has a feature where the buyer and seller can email each other using auto-generated, anonymous email addresses ([email protected]). These emails go to the buyer/seller's main email accounts, but they strip the messages of any headers/identifying information. In effect, these emails through Craigslist are anonymous.
Is this something we could utilize for research? Could we have participants email a generic email address that would allow both the researcher and participant's real email address to be redacted, yet allow for communication?
Has anyone else tried something similar?
I had a questionnaire with 18 close-ended items about factors of students' failure in English courses.
At the end of the questionnaire I asked respondents an open-ended question:
Why did you fail English in the previous semester(s)?
I made it optional and only 27 out of 56 responded to this question.
Was it correct?
I want to use the contingent method, is there any researcher have used it and can give me an idea regarding the methodology and required data for this method.
Hello everybody! I have 3 research problems and I will be using Colaizzi's method of data analysis. I am fairly familiar with the steps of Colaizzi's method but I do not know how to start it. Do I analyse my transcripts as a whole to get a final thematic map and be able to answer the 3 research problems or do I have to sort out the significant statements relevant to each research problem and then later arrive to 3 different thematic maps?
I'm trying to make these similar concepts clear in my mind but it's not easy. Let's make a brainstorming together!
Although there are some differences the related literature says:
the intent of a meta-analysis is to aggregate the findings of quantitative studies and analyze this for a true effect;
the intent of a meta-synthesis is to synthesize the findings of qualitative studies and reinterpret the data;
On the other hand, when we need to examine both the quantitative and qualitative studies, we have to prefer a different method. However, what is the the correct option for us?
Is examining both the qualitative and quantitave studies at the same time a wrong approach?
Do we need to use meta-integration (aka mixed-meta synthesis) or systematic review? What are the differences between these two methods? What are the procedures that we need to follow?
Of course there are lots of studies in the literature but lets make it clear: which one is the "right" one? Let's share, discuss and try to make a consensus on this issue!
I have some selected samples with basaltic in composition and vesicular texture. All of the primary constituents are fresh, and they don't have any alteration in thin sections. However, their vesicles typically filled by zeolite, carbonate, and other secondary minerals. I want to do a geochemical analysis for them. So I want to know, there are any effective methods to eliminate these secondary minerals, especially zeolites?
Looking forward to your valuable replies. Much appreciated!
I would like to remark this to all are interested in publishing in Elsevier:
“European-Elsevier Scholarships”, \"Request for Papers\", \"Editorial/Reviewer Appointment\" & \"Manuscript Submission\"
- Fraudulent emails in circulation.
Please note: these are not Elsevier requests!
For further infos: http://www.elsevier.com/wps/find/authorshome.authors
I was doing a PCR for influenza viruses ( more specifically defective interfering segments) and i had good results beside 2 segments. I store them at -20 degrees and i was thinking to run them 5 cycles more ( initially all the sample were run for 15 cycles) . Do i need to make fresh samples or i can just reuse the 2 stored samples. I'm thinking just to place them in the PCR machine and add 5 more cycles to this 2 samples .
Will this work ?
I know the buffer, dNTPs , Taq polymerase and others are limited and have a specific life expectancy of PCR cycles, but 20 ( 15 already done + 5 which i will run )cycles from my presumption will not exhaust all the dNTPs and other reagents.
Thanks a lot ! Sorry for this stupid question !
What is Connolly surface method all about? I have found that It is mostly used to determine the surface volume of molecules.
How can this method be used to determine the size and volume of a nanocluster?Is there any software from which Connolly surface can be plotted and surface volume and size can be estimated?
I am trying to culture P. aeruginosa (PAO1) biofilms in 96-well plates. I have tried pipetting off the media, wicking it up with blotting paper or tapping it out. All of these methods results in severe disruption of the mucoid growth.
Can anyone advise on a successful method for removing the media, leaving just the biofilm?
If you build your own corpus to address specific research questions, which method to you use to make sure It is saturated? I'm interested in methods as I work on digital data and I wonder which method is more efficient and less time-consuming.
I work in microbiology laboratory in a Flavors company, We conduct many microbiological tests to detect microbial hazardous such as E.coli, Coliform, Yeast & Mold and Salmonella.
Recently we received sample From LGC standards (used for proficiency testing )
In order to detect Salmonella we preformed the following procedure:
1. I Prepared 225 ml of buffered peptone then i added 25g of the sample to the buffered peptone.
2. Incubation at 37C° for 24 h.
3. 0.1 ml of sample in puffered peptone were added it to 10ml of Rapport Vasilidis (RV)
4. Incubation at 42 C° for 24 h.
5. Streaking plates of XLD and Brilliant Green (BG).
The results of XLD plates was strange because the grown colonies appears yellowish in color as in picture (B) not black colonies as it supposed to be.
Regarding BG results were also confusing because the media color has changed to pink but the colonies color is a bit different from typical salmonella colonies picture (A).
So to confirm this result I prepared another Fresh XLD and then I preformed streaking on XLD plates from (BG) and from (XLD) plates which used first time to detect salmonella.
The result in the end confirmed that the sample contains salmonella as in picture (C) !
So I have a situation here because XLD and BG firstly (A&B) didn't give me the confirmation that the sample contains salmonella due to the difference in morphological characteristics, but after streaking on another XLD it's confirmed that the salmonella is percent.
I think that's may happened due to decomposition of some medium components because we let the medium on hot plate till boiling = (over heating)
Is this thinking reasonable or it may be another reason for this issue ??
If you have any informations regarding this issue please share it with me.
Many thanks in advance for your support... 🙂🙂
I am struggling to find any good summer/fall/winter research school on mixed methods research (for business and management, the area of broader social sciences research - also OK). Ideally, it should be within UK and EU institutions/universities. Do you have any ideas/suggestions? Thank you!
Making CLEAs. Hi all this is my first time on the forum so not sure how this works.
sorry this is so longwinded:
I am working on a project comparing the reactivity of L-aminoacylase with a HIS tag, at different purification stages, and in immobilised (CLEAs) or free enzyme form.
I have had problems trying to immobilise the enzyme. (I have followed a procedure from Toogood 2001 for making CLEAs which was adapted from Cao 2000).
This involved ammonium sulphate precipitation (I used 80% concentration) of the solution containing aminoacylase. It is stirred overnight at 4°C, followed by slowly adding 1% (v / v) formaldehyde and leaving under the same conditions for 2 hours. The subsequent washing step on filter paper from the protocol would never yield any protein, so I now centrifuge the precipitant first to get a pellet, I then I wash and filter the pellet and dry it in a desiccator.
However the majority of the pellet is again washed away, which leaves me with concerns that the protein has not been properly cross-linked.
I only have One week left of my project to try and get good CLEAs. Has anyone come across any similar problems?
Being an academic of finance and accounting subjects, I always look for new and contemporary ideas, thoughts, research, methods, models, processes involved with the research in the broad area of finance and accounting. once I found a website containing researches in the last 10 years, but unfortunately I lost it in the bookmarks.
Can we share the sources for getting such resources for learning and enrichment of knowledge in Finance and Accounting?
Recently, I got problems about Pichia expression. My target gene was inserted into pPIC9K vector via EcoRI and NotI restriction sites. And then the SacI digested construct was transformed into GS115 by electroporation. More than 200 transformants were obtained from the MD plate, and whcih was collected and subsequently plated on YPD plated with G418 gradient(0.5mg/ml to 3mg/ml). Only a few colony formed on the high G418 concentration plate, and then the colony was streaked on new G418 plate for isolation single clone. Then the genome DNA was extracted and PCR was performed.
PCR employed 5'AOX1 and 3'AOX1 primers gave 2.1Kbp bond, indicated the AOX1 gene from Pichia was still remained. however, PCR with my target gene speical primers, no bond was obtained. And subsequent western blot analysis shown that no target protein was expressed by induced expression.
Help!!!Where am I going wrong?
When should I change the water including antibiotics cocktail for antibiotics-induced microbiota depleted mice (AIMD mice)?
Hello, fella researchers.
I am trying to make AIMD mice for my study.
I have seen many papers to find method.
The antibiotics cocktail including ampicillin (1 g/L), neomycin sulfate (1 g/L), metronidazole (1 g/L) and vancomycin (500 mg/L) will be dissolved into the sterile drink water and provided to mice for 4 weeks.
But, I can't find when should it be changed. Every once in two days? I can't find.
So, If there is anyone who knows this or did this before, please help me.
I am trying to revive my cells and I don't know what they're being affected by. I have thoroughly cleaned every corner of the culture room and incubator; done all possible cleaning practices to avoid all possible sources of contamination. Now my cells are not adhering to the surfaces of the plates. They become rounded and are floating in the media. What additional techniques can I try to establish my culture? I don't know whether it is some biological contamination or some physical factor that is playing a major role.
I'm doing research on fault detection ,classification and identifying ,I have found out fault detection using wavelet analysis need a guide about other methods.
I am looking for methods of measuring of the Seebeck Coefficient or devices alternative to Netzsch SBA 458 Nemesis. It will be great if the simultanous analysis of Seebeck Coefficient and electrical conductivity would be possible.
I am writing review articles in which I want to describe procedures from other papers.
Indeed, It is clear that the origin of data must be listed. But how detailed can a procedure from a foreign source be written down - in own words? Especially when specific procedure parameters (e.g. concentrations of chemical components, temperature etc.) are extracted from a paper - are there dangerous copyright traps i need to consider?
Any help is highly appreciated..
I am aiming for hyaluronic acid-based microgels particles, which are homogeneously modified with DNA. Therefore, SH-modified DNA, which is additionally functionalized with a fluorescence dye is couple to a PEG-maleimide crosslinker and mixed with hyaluronic acid to from W/O-droplet-emulsion. By following the gelation process using fluorescence microscopy, I observe diffusion of my DNA (around 1200 bp) to the W/O-interface giving particles with DNA only at the surface (like a corona). The only thing that I can think of is that this might be an issue of electrostatic repulsion between polyanionic hyaluronic acid and the DNA during the gelation process. Can this effect be avoided by adding additives to the emlsion, like CTAB or something else that lowers the repulsion? Or do you have any other reason, why I oberserve this and how to avoid it?
Thanks for your help !
Has anyone dealt with technical issues with both primary rat neuron/glia co cultures and human iPSC neuron astrocyte co cultures (both on PDL), where the cells seem to ball up near the cell body and cause long filament string like processes to one another? We think it could be due to the coating or a cell signaling process? See image comparison of healthy vs. "spindled" like cells. Apologies for the bad quality image due to uploading on here.
Any technical advice welcome. Thank you!