Solubility - Science topic
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Questions related to Solubility
Dear colleagues, i am working on quercetin but i found problems in solubility. i dissolve it in DMSO for making stock solution (30 mg/kg) then i made dilution in PBS buffer. unfortunately, the drug precipitated. i could not use DMSO (100%) for injection in vivo as it has some adverse effects. Did anyone have this issue before and how to solve it. thanks in advance
I'm trying to make a solution for haptoglobin measurement using this product. My method gives me the following directions:
0.6g o-dianisidine, 13g sodium phosphate monobasic and 0.5g EDTA dissolved in 1l deionised water, pH adjusted to 4.1.
I know that o-dianisidine is only slightly soluble in water (60mg/l) but possibly more soluble under acidic conditions however it still did not totally dissolve when at pH 4.1
If I dissolved in alcohol, approx (50ml) does anyone know if the addition of the alcohol to my buffer will affect the assay result ?
Anyone have experience with this colorimetric assay for haptoglobin? solving the same amount in
How do I estimate the number of molecules of drug that will be loaded in my dendrimer formulation by phase solubility studies ?
How can we say whether the solubility of Ar is more or less than CO2. To be precise how many times more or less is the solubility of Ar in Sea water than CO2?
I am a phd research scholar working on cilostazol. I have to give cilostazol i.p route. it is available in the form of powder which is soluble in chloroform, ethanol, methanol.
please let me know .
I am conducting a CO2 electrolysis experiment in aqueous medium, where I will be stirring the medium with a magnetic stirrer. I read in the paper linked below that a perpendicular magnetic field (to the surface of the electrode) of more than 1 T can improve bubble dispersion and thus solubility.
As I am stirring my medium with a magnetic stirrer, I was wondering whether such effect would apply to my system. But I am not sure about the strength and direction of the magnetic field generated by the magnetic induction stirrer. Is it ≥ 1 Tesla? And what is its direction?
Dear RG colleagues,
Can you guys please give me some tips to dissolve Devazepide (CCKA(1) Receptor inhibitor)) to inject safely with correct dose to mice?
I saw that it was well dissolved to DMSO but when I further add PBS, it become hazy.
Adding Tween 20, 80 or PEG my increase the solubility of Devazepide but they are not yet available in our lab and also concern about the safety and side effect for the mice.
Thank you very much in advance for your kind help!
I can calculate the solubility of a polymer molecule of small molecular weight at different temperatures. How can I determine the solubility of the same polymer having a large molecular weight with the same result? Is there any conversion formula?
Hopefully this is not a stupid question, but I have problems finding information about this. This question is especially targeted for adhesives. A relatively large amount of NaOH is needed to catalyze the polymerization reaction between phenol and formaldehyde. As it only functions as a catalyst, it is not consumed. When making plywood, what happens to the NaOH? Is it simply trapped within the polymer matrix, or is is pushed out of the curing polymer as its solubility decreases? Is it not problematic?
i will examine the soluble form of negative immune checkpoint inhibitors ....
what the function of membraneous PD-1
and what be the function of soluble PD-1????????
and as well as
what the function of CD4 membrane bound and soluble form of CD4 what be the function in peripheral circulation?
is presence as a metabolic process or cell aging process or its presence have a unique purpose? what regulates the level of membrane form and soluble form for any immune marker
I'm following the instructions here to create a 3D printable bioink but after i freeze dry my samples, they only partially dissolve in water. I tried heating with a stir bar too and it would still not completely dissolve. In the paper, they describe their Sil-MA composite as a powder after freeze drying but my sample is more similar to a strong Styrofoam. I cannot grind it up with a mortar and pestle and cutting it is very difficult. I've had relative success dissolving it in formic acid but but i think the acidic environment hinders crosslinking so I'd perfer to use water as the solvent instead. Any ideas?
I'm looking for molecules (molecular weight > 400 g/mol) soluble in both water and non-polar oils, such as cyclohexane.
I hope the molecule is partitioned in each phase (water and non-polar oil).
Please let me know if you know any of them.
I'd like to prepare a solution of MoCl3. According to the supplier, MoCl3 is soluble in nitric acid and sulfuric acid, and insoluble in water and dilute hydrochloric acid.
Does anyone work with MoCl3? I'd like to prepare a solution of MoCl3 with a concentration between 0.1 and 1 M but not using nitric acid as it will give (MoO4)2- species. I need to obtain the Mo3+ species.
I have a sample soluble in water. To carry out the Thin Layer Chromatography (TLC), which organic volatile solvent is recommended? It is also soluble in DMSO, however DMSO is not a volatile solvent. It is also not soluble in Ether, DCM, EA, EtOH, Hexane, CHCl3, etc. For example, the reaction is A + B --> C, here A & C are soluble in Ether, DCM, EA, EtOH, Hexane, CHCl3 but B is not, whereas B is soluble in water but not in Ether, DCM, EA, EtOH, Hexane, CHCl3. The eluent I am using for the TLC experiment is Hexane:EA. Two maerials (A & C) are responding in the Hexane:EA = 2:8 to 5:5, however B is not. In fact, B is not responding in Hexane:EA = 2:8 to 9:1. Kindly suggest. Thank you in advance.
I'm trying to improve the solubility of butylated hydroxytoluene (BHT) in common vegetable oils (soybean/sunflower) in an liquid product to be used at 298 K. So far I've tried the following:
1) Formulate an emulsion (unstable in time and you need specialized equipment)
2) Reduce viscosity of the solvent by adding a cosolvent like a fatty acid (C18, no luck)
Knowing that the solvent has to be a food-grade oil, could you please suggest any ideas or recommendations to choose a suitable solvent for this application?
If yes, kindly share energy input values and carbon emission equivalents for biological sources like Phosphorus solublizing bacteria (PSB) and AM fungi.
I've been looking around to find something to tell me what will dissolve 1-octodecene? But I've not found anything real conclusive.
I did find something that said it was soluble in Alcohol, Ketones, et. all. What I'm interested in knowing is just how soluble is 1-ODE in 2-propanol, or Ketones.
I don't really have access to said chemical or otherwise I'd check myself.
I want to purify my insoluble organic compound from sodium chloride through washing.
I have done my first trial with distilled water having tds approx 44 p.p.m and the second with fresh ground water having tds approx 500 p.p.m.
The results differs in both trials.
So how t.d.s and hard minerals affects the solubility of sodium chloride in water?
how can i calculate the ratio of solubility based on water quality (t.d.s).
Suggestions are highly appreciated.
Hello, I performed Plasminogen gelatin zymography on total soluble protein extracts and purified samples of tissue plasminogen activator. I was expecting more activity of purify sample than total soluble protein but I got less activity of purify samples! Anybody with this experience?
The attachments is Zymogram gel.
Thank you for your guidance
I am incorporating di/tripeptides into an anhydrous reaction carried out in either DMF or DMSO but have encountered solubility issues with the peptides I have used so far, being far more water-soluble. Which amino acids typically confer high solubility in either of these solvents?
I perform subcellular fractionation experiments (cytoplasmic, soluble nuclear fractions, and chromatin-bound fractions) to see the effect of different treatments on my proteins of interest, which are between 32-37 kDa. I use alpha-tubulin for cytoplasmic fraction and H3 for a chromatin-bound fraction. Still, I didn't find a good marker for the soluble nuclear fraction to be more or less than 32-37 kDa. Please, if could anyone suggest to me which soluble nuclear markers could be useful in my case.
The solubility of my PVDF has decreased somehow and I believe this is due to changes in the molecular weight on the PVDF.
After 24 hours i'm finding my PVDF is not dissolving in room temperature mixture.
How do I increase solubility if I am not able to increase temperature as it will affect the characteristics of the end result PVDF after the process, I can't increase the shear rate of the mixing as this will increase the temperature too much.
Is trying to increase the solvent volume or concentration the only option to toggle with?
I'm looking for a simple method (spectrophotometric , precipitation....) for routine measurement of copper in wastewater that contains 100 to 1000 mg/l soluble Copper
When the Cu(II)Cl2 is dissolved in ethanol:DI water (70:30, v:v), the solution becomes turbid. Normally, Cu(II)Cl2 is soluble in ethanol and DI water, but it is not 100% soluble in ethanol:DI water mixture.
I am investigating the solubility of PVDF in a solvent. I have reached at point at after 18-24 hours the PVDF is still not fully dissolved in the solvent.
Unable to change the solvent, I'm wondering the effect sonication may have on the speeding up the dissolution process. While this will increase the dissolution rate, i'm wondering the effecting it may have on the dissolved PVDF. will it effect the characteristics?
Also would the sonification just reduce agglomeration? And instead would it just achieve increased dispersion in the mixture instead of preferred full molecular dissolution?
Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will require a large database of the retention time and other chemical properties such as logP, polarizability, dipole moment or intrinsic solubility in water of between 100 and 1000 molecules. these molecules will constitute the training set. I was therefore wondering whether you know of any databases which contain data on retention time and various other chemical properties.
I am trying to test Anthracene (An) on the cells and I could not dissolve An in buffer solution and even in ethanol the solubility is very poor. DMSO is not working as well! for my experiment I prefer using buffer solution HBSS , ehanol or DMSO (generally not more 1% from ethanol or DMSO). How can I dissolve anthracene and prepare the dilutions without using Ethanol or any type pf alcohols or with a tiny amount of ethanol not exceed 1%?
I'm exploring the world of emulsions, but I'm not sure if it is the correct way to solve my problem.
I wonder which solvents the linear polyglycerol(PG)s are soluble except water, methanol?
Or is the linear PG soluble in ether? I heard that it has partial solubility in ether. If so, can i precipitate the PG with MeOH/ether?
Can any give suggestions regarding to the calculation of estimation of Total soluble sugars or total carbohydrates by anthrone method.
Please help me
I am currently searching for literature about amino acid propensities to exclusively form certain secondary structures regardless of what kind of peptide or protein do they form (e.g. membrane, cytosolic, soluble, globular protein etc). What are your thoughts on this one?
May you also recommend literature to support your answer. This would help me give a mechanistic basis of secondary structure formation from a given set of amino acid residues.
Thank you very much.
I removed the axial chloride by cyanide it shows significant color change and as well as strong cyanide signal in the IR, however I tried several recrystallization conditions but unable to get the single crystal, my complex has PZPY system and solubility in DMF and Methanol is quite good and partially soluble in other ACN and THF , I tried most of common solvent however, unable to grow the crystal, if anyone has good suggestions or ideas are much appreciated!!
Thanks in advance
I am working on a project which requires me to know the concentration of the gas soluted in the liquid.
I know the solubility(900 [H2]/M and 1.387 g/ml), Henry's constant(370 kH/MPaa) and Viscosity(85 cP). Additionally, the pressure is 1 atm, the temperature is 273K, the gas consists of 1% H2 and 99% N2 (v/v). Since the gas is continuously put in by a digital mass-flow controller, so the partial pressure of H2 can be considered as constant.
Can I calculate the concentration of H2 in this liquid based on the information above?
For example, if I get some pure ethanol (99%) and dilute it down to 70% (let's say I add 700uL ethanol and 300uL water to make around 70% ethanol) can I use this to make up my compound?
I have a vial of powder containing 200 units/mg protein. The solubility is 1mg/ml H2O.
Then if I add 1ml of H2O, I will have 100 units/mg protein stock.
Overall, I need some 20 units/mg aliquots for my experiment.
How can I achieve this? Also, is it correct that I add 1ml H2O to 200units/mg protein I will get 100 units/mg?
i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc..
I already tried benzene (no solubility), toluene (no solubility), DCM (no solubility), Chlorbenzene (maybe slightly soluble) and Flourbenzene (maybe sligthly soluble).
Any suggestions are welcomed.
I have different OD values and different tissue weight.. and when calculating it the lower tissue weight content the greater value thn the higher tissue weight !! I couldn't get the proper results..can anyone help me to find out results!!??
I want to compare my result with other studies.
Exchangeable Al (Al3+) in this analysis using cmol (+)/kg that equal with meq/100g. However, many works use the different unit on presenting the exchangeable Al values.
A. µM as a unit for soluble Al.
For example (Cristancho, Hanafi, Syed Omar, & Rafii, 2014) reported at low pH 4.38, a total soluble Al value was 251.9 µM = 251.9 µmol.
Molar mass of Al = 26.981539 g/mol = 26.981536 mg/mmol = 26.981536 µg/µmol.
The total soluble Al = 251.9 µmol x 26.981536 µg/µmol = 6,796.64892 µg/kg = 6.80 mg/kg
6.80 mg/kg = 6.80 mg/kg: 90* = 0.07 cmol (+)/kg
So, the total soluble Al 251.9 µM = 0.07 cmol (+)/kg.
B. Al in mmol (+) /kg.
(Gomes, Gonçalves, Rocha, & Menegale, 2019) reported that in pH 3.9, the values of Al was 17 mmol/kg
17 mmol (+) /kg = 17 cmol (+)/kg: 10 = 1.7 cmol (+)/kg
C. Al in ppm
(Haug & Foy, 1984) summarized that aluminum become soluble at pH< 5, at concentrations 1 and 30 ppm.
ppm = mg/l = mg/kg
30 ppm = 30 mg/kg
30 mg/kg: 90* = 0.33 cmol (+)/kg
Note: *converting mg/kg Al to cmol (+)/kg by divide mg/kg Al by 90 (source: https://www.dpi.nsw.gov.au/__data/assets/pdf_file/0007/127276/Chemical-tests.pdf).
Is that correct?
A part from cellulose acetate and pmma, what polymers are out there that are not soluble in water, but are soluble in ethanol, methanol, acetone, or low toxicity solvents? I'm avoiding chloroform and acetic acid!
Hi, I'm investigating the solubility of a substance (A) in a solvent (B), by repetitive addition of small quantity of A in to B and shake the mixture.
I already reached the stage where 24hr shaking could not fully dissolve A. But when I sonicate the mixture, A fully dissolved! This was repeated several time.
To my knowledge, sonication only speeds up the dissolution process, not increase the solubility of a substance. Or is it really the latter?
We have developed drug/co-former by co-crystal formation, in this case, we don't know how to distinguish the co crystal whether in really co-crystal formation or salt formation??
In solubility, both of them can increase the solubility of drug, meanwhile the thermal analysis (TGA and DSC) showed shifting higher of melting point peak (the melting point of drug is lower than that of co-former)
Is the rate of dissolution of salts in water directly related to their solubility?
The solubility of NaCl and KCl in 300C are 36 and 34, respectively. If 1 liter of water is added to 1 kg of a mixture of these two salts (NaCl:10 %w.t, KCl: 90 %w.t), how will the liquid and new solid composition after the dissolution?
In other words, how is the equilibrium composition of the solution estimated in this case?
I'm trying to improve the solubility of curcumin (a plant extract) for an experiment by creating an inclusion complex using Hydroxypropyl-β-cyclodextrin.
I have decided to create the complex using a magnetically stirred physical mixture of distilled water, Hydroxypropyl-β-cyclodextrin, and curcumin at 50 Celsius. I will then add acetic acid to bring the PH to 3 as this seems to be PH/temperature that creates the strongest complex in studies.
How strong is the cyclodextrin complex? I am thinking of using a low temp dehydrator to dry the mixed solution, and then a grinder to create powder. Will this destroy the complex significantly - and if so how can I preserve the inclusion complex while creating a powder?
I am working on the design of the RO system for mining wastewater. I have 115 ppm of BOD in feed. I am always confuse about what should be the maximum limit of BOD in RO feed. Majority of big companies like DOW specifies 5 - 10 ppm of max BOD is allowed in RO feed. However, they are not specifying that whether it should be soluble BOD or insoluble BOD. If we have 100 ppm of soluble BOD than I don't see any reason why we can not use RO. If it is insoluble; than it depends on particle size. I would highly appreciate if some one can give me information about what should be the maximum BOD/COD concentration in RO feed and which type of BOD / COD is allowed? (like soluble, insoluble etc.). Also BOD and COD are generalized terms. There are many compounds fall in this category. Does someone has some type of article or document that includes different type of BODs and CODs and tell us whether they can pass through RO or not.
I am working on synthesis of heterocyclic compounds containg l-tryptophan. Most of the synthesized derivatives aren’t soluble in Dmso , I tried to use a mixture of TFA and Dmso-d6 , sample was soluble but the peaks wasn’t clear, Does the color of sample have any relation with clarity of the spectrum???
I need to calculate solubility constants for some minerals, I intend to use them for geochemical modeling. I've searched for these contants in EQ3/6, Thermoddem and SUPRCTBL, however some minerals are missing on the databases, for example Andesine, which is one of the key minerals on my simulation.
I have seen that in some papers, the the log K values are derived using SUPCRT or EQ3/6. The authors use the mineralogical characterization (from microprobe data for example) and theoretical formulas, either using a solid solution approach or directly, however the detailed methodologies are not explained. The available information in this regard is also scarce. I am able to calculate log K values for defined minerals (in the databases) in SUPRCTBL but I do not know how to calculate the values for new minerals.
Any advice in this regard is warmly received, bibliography, tutorial......
Thanks in advance.
I am performing an experiment about the biodegradation of insoluble substances.
We first dissolved this insoluble substance in acetone to be a stock solution, then we add this stock solution to a liquid medium to stating a 14 days bacterial degradation test.
But the acetone in the liquid medium will volatilize as the experiment goes on, then the insoluble substance will be precipitated in the liquid medium.
therefore we can't analyze the concentration of the insoluble substance in the liquid medium perfectly.
How can we do to evenly sample the insoluble substance in the liquid medium and do not affect the bacteria growth simultaneously?
I would like to calculate the solubility of carbon in metals ( mono-metals e.g. Ni, Co ,Fe, Mo and Cu) and mixtures of metals ( e.g. Ni-Fe, Fe-Co & Fe-Mo) at 700 deg C. The major focus is on transition metals and mixture of transition metals
Is there any way by which I can calculate solubility of carbon in all transition metals and mixture of metals ?
is there any commercial database/software ?
I contacted Sigma Aldrich technical support for oxonic acid potassium salt solubility, and I was informed that it dissolves in water (60mg/ml). I tried in water with constant heating and stirring but can't dissolve it. Any suggestions will be highly appreciated. Thank you in advance!!!
Please provide me citations or research papers regarding to biochemical parameters of total soluble sugars, Total Protein, Starch.
In a lecture, we were discussing the octanol-water partition coefficient. I understand the concept, why it is used, how it is used but our instructor asked "what do you think about why we are using octanol for this coefficient?" Immediately, I thought solubility, polarity, availability but she did not give us an exact answer. So, if there is a reason why we prefer octanol for this test, I would like to hear. Thanks in advance.
I am producing polymers, and I made a solubility test and swelling test, this polymer gave the highest solubility results and it did not lose its shape, and the highest swelling ratio also ??
what is the scientific explanation for this case??
Concerning the dissolution testing, I don't understand why we work in NON-SINK conditions when doing development (for example if I test the solubility of my active ingredient) and in SINK conditions when we have to do regulation...
I have synthesized one new fluorescent polymer containing high electron donor-acceptor as well as hole transporting moieties. It has an excellent ability to form thin film-like paper (which has insoluble in water, brittle in nature) without adding anything. It is quite soluble in organic solvents and showing solvatochromism property as well. Can anybody suggest that where can I use this one?
I need to find experimental data for CO2 solubility in water that can be simply modelled by using the Peng Robinson equation of state for a project. The concentration of CO2 has to be very low for this purpose. I wonder if you could suggest to me a research article that contains the described experimental data for my project?
I need to find experimental data for CO2 solubility in water that can be simply modeled by using the Peng Robinson equation of state for a project. The concentration of CO2 has to be very low for this purpose. I wonder if you could suggest to me a research article that contains the described experimental data for my project?
If we are to add 10g each of TiC, TiN, NbC, ZrO2, TiB2 in a matrix let say Austenitic steel, I would like to know if the solubility of each reinforcement in the steel to form a composite could be predicted using software and how it could be done.
I have a soluble yeast protein that needs to be transported to mitchondria for processing. I have put the Cox IV presequence infront of the ORF. I know it would be synthsized in cytosol, imported to mitchondria where its presequnce would be processed and it would go through folding with the healp of mtHSP70. My question is: would it be realed into the cytosol after the folding?
how can i dissolve both the anionic surfactant ( SDS ) and salt ( NaCl ) in 5 ml water without precipitating salt or surfactant or sticking to the wall or bottom of the beaker?
I need to dissolve 6 and 9 g of PVC in 100 grams of MEK each. I've left the solutions to shake over night but the PVC did not dissolve. I increased the temperature to 50 Celcius to see if heat has an effect but it does not seem to work. What do I need to do to completely dissolve PVC in MEK?
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs works as a drug carrier which it is also known to be soluble in our blood.
If the drug is water insoluble, this means it cannot be dissolved in water. In this case, we need to melt the drug and mix it with lipid (heat them above 5 - 10 C of the lipid melting temperature) and then, mix them with the aqueous solution (surfactant + water). Hence, we will have an emulsion.
Was wondering, what is the state of the drug (eg: drug is solid at room temperature), if the melting point of the drug is higher (for instance 200 C) than the melting points of lipid (70 C) and surfactant used? Is it dissolve in the fluid or is it a dispersion?
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the max concentration?
The same question about solubility in ethanol.
We are Phthalo Pigment manufacturer. Soluble Copper is present in our by product spent acid. If we remove or reduce the amount of soluble copper from the spent acid, it could be easy to dispose as by product in market. It will reduce our treatment cost.
When I am performing the solubility studies of my drug in different solid lipids for making SLN, the solid lipid is becoming solid immediately after removing from heat condition. So, I am unable to check the concentration of drug solubilized in the lipid.Please help me in this regard.
UPDATE: the temperature sensor of the pH meter was broken
I use this buffer during my protein purification process:
20mM Tris-HCl pH 8.0, 6M urea, (+ protease inhibitors)
In one step of the protocol, I have to lower the pH to 2.5 (my protein is soluble at this pH), ultracentrifuge, and then raise the pH to 8.5 again. I use HCl and NaOH to adjust the pH and I do it on ice. After a while, the pH drops from 8.5 to around 2.5-4.
I did a test and took buffer without a protein and measured it at two temperatures. On ice, it was ~3.5 and at 25C it was 8.5. Is there something wrong with the buffer or the pH electrode?
I just can add that my colleague had a similar problem with Tris buffer at pH 7.5.
Thank you :)
I synthesized a conjugated polymer, but that's a little bit soluble in common organic solvent.(ex. MeOH, hexane, chloroform... etc.)
Except for soluble part, there remain product a lot.
Souble part can be characterized by GPC(Mn is about 1800.).
I know that the soluble part also exhibit low M.W that's hard to called polymer(monomer's M.W=170g/mol)
but, I wonder know insoluble part is polymer.
How can I confirm that?
I really appreciate your reply.
Hello everyone ,
Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating .
I have tried salting out by electrolyte solution to reduce solubility but I am not sure of the results.
Thank you in advanced.
After seeking for references, most of the solvents for Dess-Martin is DCM, but my compound has a poor solubility in DCM. Because my compound is acid-sensitive, I prefer not to use IBX to oxidize my compound to aldehyde. I am wandering if DMP in DMF would produce side product or oxidize my compound to carboxylic acid? Thanks for help.
Upon some research I found different protocols of BPA solubility, some state a 5 v/v % ethanol (MOTOYAMA et al ) will catalyze the process of solubility and BPA will stay in solution (being an azeotrop the ethanol won't evaporate). The European Union Risk Assessment Report CAS: 80-05-7 EINECS No: 201-245-8 states that BPA solubility in water is 300 mg/L so does pubchem. How can I get a solution of more than 300 ppm BPA completely dissolved in water preferably without ethanol? Will dmso work as good? Waiting for your replies. Thank you
I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it doesn't. I have little experience, maybe I am making some mistake?
I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found that many papers using 2 M TFA for this analysis. I also have tried using 1 M H2SO4 at 100C, but it was failed too. Please, any suggestions, what other methods should I use for this analysis? Many thanks.
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble
any suggestion, please
My n hexane plant extract is not soluble in any solvent. I have tried water, normal saline, tween 20 (2%, 5%, 10%, 50%), tween 80 (2%, 5%, 10%, 50%), pure DMSO, ethanol (2%, 5%, 10%, 50%, 97%), infact n-hexane itself as well. But it's not completely soluble in any of these. I have warm these and vortox as well but no positive results were observed. Sparingly soluble status is there.
Plz suggest some appropriate solvent to dissolve it fully and minimal toxicity as I have to perform in vivo activities and mice dies with DMSO and other issues are also encountered with cell lines studies.
I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it in pbs/ media which is leading to precipitation. I am aware that i can prepare highly concentrated stock, but because its an expensive compound i cant do that either, is there any other way that i can go ahead with?