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I was simulating RPL on cooja for mobile nodes and faced the error statement below, because of which the collect view did not collect the network data. Can anybody explain the reason and how to resolve it:
[java] void: *** IOUnit read from non-existent IO at $0010
The screenshot is also attached for the same.
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This stack flow discussion might be helpful, have a look
Kind Regards
Qamar Ul Islam
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I was checking Ag-Ab interaction using biosensor (APS)and then I got a association dissociation curve that increasing at both steps and so I need to explain that if there is available reason.
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My first thought here is a buffer mismatch. Have samples been dialyzed to the same buffer, and is the buffer consistent between wells used for different steps?
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I have been simulating pipe flow with velocity inlet and pressure outlet system. I am using LBM. for some reason the pressure at the inlet (if we go from 1 to 2000 in length pressure at lattice 10) is varying from one iteration to another for shear thickening fluid with power law index greater than 1.3. what could be possible problem which I can fix to get a constant pressure at the inlet in radial direction.
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@R.P. Peçanha no it didn't happen for newtonian fluid.
And simulation is starting with flooded lattice
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Hi,
I have simulated protein-GTP complex using Gromacs-2020 and CHARMM36 FF for 250ns. I analyzed binding energy between protein and GTP using g_mmpbsa tool. As we know that GTP binds very strongly to proteins (binding energy should be in high negatives). However, I am getting positive binding energy between protein and GTP for unknown reasons. when I checked the summary file, as shown below, it seems polar solvation energy is the reason behind positive binding energy. Here I am also attaching input files I used for your perusal. Thanks.
SUMMARY
===============
van der Waal energy = -189.277 +/- 20.435 kJ/mol
Electrostatic energy = -184.267 +/- 93.292 kJ/mol
Polar solvation energy = 1234.921 +/- 63.714 kJ/mol
SASA energy = -19.393 +/- 0.848 kJ/mol
SAV energy = 0.000 +/- 0.000 kJ/mol
WCA energy = 0.000 +/- 0.000 kJ/mol
Binding energy = 841.983 +/- 55.707 kJ/mol
===============
END
===============
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Increase Solute dielectric constant (pdie=4) in pbsa.mdp file and rerun.
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I am using DH5 alpha recombinant cells with ampicillin resistance gene but growth of cells is very slow. I am using 50ug/ml ampicillin. what may be reason for that?
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It took about 10-12 hours for me to get colonies on my plate, so I transformed the same plasmid with the same insert again, and this time I got a good number of colonies on ampicillin agar medium, but when I subcultured those colonies in ampicillin and kanamycin LB medium, they didn't grow, whereas I got good growth in only ampicillin medium.
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I am studying MH curves of CdO nanoparticles at room temperature
magnetization saturate at low field but at higher field (around 20000Oe) magnetization decrease. The possible reasons behind this are:
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Dear Leelavati Thakur in addition to the relevant reference suggested by Mohamed El Hafidi please also have a look at the following potentially useful articles which might help you in your analysis:
Revealing a room temperature ferromagnetism in cadmium oxide nanoparticles: an experimental and first-principles study
and
Room-temperature ferromagnetism in nanostructured submicron Cd/CdO particles
The second article has been posted by the authors as public full text on RG. Thus it can be freely downloaded as pdf file.
Good luck with your work and best wishes!
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I used 2X Phusion Master Mix during my PCR reaction and after that I run the PCR product (10ul).
I can observe a faint band but I do not why it looks like degradation. I attached a picture to ask what could be the reason of this big smear on the gel?
Thanks a lot for your help.
Mary
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Surcharged wells with DNA. You could load less volume (3-5 ul) or even dilute your PCR products.
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Calcination typically increases the aggregation and size of nanoparticles. However, are there any exceptions? I have synthesized NiO/CuO nanoparticles. After calcination at 300 °C the size and aggregation of nanoparticles decreased somewhate (FESEM images). Are there other reasons for this?
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Thanks, Swick. your description makes sense. This experiment was repeated twice: the particle size was somewhat reduced (maybe just a small fluctuation), but the aggregation was clearly reduced. May I please know if it may be related to calcination? Is there any citation for this?
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I have published in different journals of materials science area from Elsevier, Springer and IOP. In general, the peer-review processes have taken from 6 up to 12 weeks (in the worst of cases). Is this time range reasonable for a peer-review process considering a subscription model publishing?. Are these characteristic times associated with this publishing model or it is similar to the case of open access?
How much time is reasonable for carrying out this process? In particular, I think this process could be much more efficient and fast. Is the review speed directly associated with the lack of monetary incentive for reviewers? Share your opinion!
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Dear Simon,
We are all running regular routines and writing a good, informative review takes time. One has to read/screen the paper, search for relevant papers, reflect about the manuscript and its value, and finally write the report. In my substantial own experience as an editor and reviewer, I noticed that the quality of reviews differs a lot and, in general, deteriorates from year to year. The standard reviewing time for the paper in times of the regular mail submissions was almost never less than 3-4 months. Nowadays many journals that take it seriously still ask for review in about 3 months time. But there are many that do not want to wait that long. I am very skeptical about journals that ask to submit a review in 10-14 days, it is very unlikely I will leave all my regular teaching, research, and administration routines aside to review the paper. As an editor, for quite a few years I receive the reports where the abstract of the paper is somehow reformulated and the paper is recommended for publication with some minor corrections suggested. I do not trust such "reports" and never use them for taking decisions. On the other hand, I immediately recognize longer, substantial and trustworthy reviews which I take very seriously but they, unfortunately, are turning back not so often nowadays...
The bottom line: I would say that a half-a-page reports written in two-three weeks should be simply disregarded. I believe that reviewers need at least 2-3 months to write a decent report and it should be definitely over a page or even two... Even when we all are eager to see our research published faster, quality should always come first.
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Hello all,
I am attempting to determine the concentration of B. diminuta bacteria in an inoculated broth after 24 hours at 30 C on a shaking incubator by performing a spot plate of diluted samples and back calculating the original concentration. When I perform this, I am consistently getting very high CFU/mL values of approx. 1x10^11 CFU/mL. Can someone look over my experiment and tell me if I am making an error somewhere or is this value correct?
1) Inoculate 500 mL of Saline Lactose broth with inoculating loop dipped in pre-prepared glycerol stock of B. diminuta. Incubate on shaking incubator for 24 hrs. at 110 rpm.
2) Make eight 10x serial dilutions from 10^-1 to 10^-8 using 5 mL in 45 mL peptone water (1 g/L).
3) Plate 20 uL of each dilution in duplicate on Tryptic Soy Agar plates, allow to dry for approx. 5 minutes, and place in static incubator upside down for 18-24 hours or until colonies are visible for count.
4) Count the average # of colonies per dilution and calculate the CFU/mL using the following calculation: CFU per ml = Average number of colonies for a dilution x 50 x dilution factor.
Example: 10^-8 dilution average colonies: 19, (19 x 50 x 10^-8) = 9.5 x 10^10 CFU/mL.
Thank you for any assistance you can offer
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As Michael said, 10E11 cfu/ml seems well beyond what one would expect.
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We are trying to do whole cell patch from culture cells. We use Axon 200B and pClamp 10 for recording. We can get a good seal, but when we try to break the cell, the cell dropped or dead. One thing we noticed is that under the CELL mode in the membrane test, the Cm, Rm, Ra and Tau all show red arrows. It looks like a large current injected into the cell. Does anyone know what might be the problem?
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Hi.. we use Axon 700B amplifier and Digidata 1550B digitizer. Pclamp10 dongle is missing is anyone can help?
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Non-renewable nature and environmental pollution toward the climatic changes are some disadvantages of fossil fuels (oil, natural gas and coal).
Hydrogen as a zero-emission fuel is a good solution to avoid the pollution. Ability of production of hydrogen fuel from the most available renewable energy, solar power (power to electrolyze) is a major encouraging point.
Trapping the countries in prize controlling authorities (OPEC) is another important reason to turn the hydrogen fuel.
However, risk of storage and handling is a major issue to be addressed when hydrogen is used as a fuel for general use.
I would like to discuss the ability of current technology for the production/use hydrogen (as a alternative for fossil fuel) for general needs.
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Our recently published review paper entitled “A review of cleaner alternative fuels for maritime transportation” might help in adressing this question: https://www.sciencedirect.com/science/article/pii/S2352484721002067
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My samples are mainly: clays and clay minerals, synthetic layered double hydroxides and zeolites.
Is there any reasonable alternative for Malvern Zetasizer (Advance or older NanoZS)?
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Hello Jakub,
There are other DLS instruments that can measure the zeta potential of clays, hydroxides and zeolites, however I understand that normally the measurement of such properties require quite dilute suspensions (to eliminate multiple scattering and give good electrophoretic mobility data). The Malvern ZS and the Advance use a non-invasive backscattering technique whereby the laser and lens can focus on the a tiny surface of the sample and get good data on even concentrated dispersions (up to 40% solids apparently). This along with some other software functions such as their adaptive correlation (which can eliminate spurious data from dust particles and the like) make it much more usable than other systems. If it were my lab, I'd be pushing Malvern for a discounted price, rather than looking at cheaper instruments.
As a full disclosure, I did previously work at Malvern although in a different product line. I hope this helps,
Best regards,
Phil
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Dear researchers,
I am new to Western blot analysis. whenever I am trying to absorb the phosphorylation of ACC (Acetyl-CoA Carboxylase) detecting by Western blot, I always observed a double band of pACC.Even several research papers observed double band but none of them explained the reason. Herewith attached the pictures of my membrane which I observed phosphorylation of ACC. Please help me to identify the reason.
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The reason for the double band may be the degradation of the enzyme Or isoforms. We normally use Phosphatase / Protease inhibitor. Do you use both of them? Please try to reduce the SDS-PAGE gel by 6% and try.
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As a result of testing the produced fibers, an additional peak was observed in the cooling and heating diagram.
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please could anyone of you dears let me know which type of DEM is better for mapping flood prone areas and why with scientific reason or reference ALOS palsar dem 12.5 m and coperinose GDEM 30 m? really appreciate you kind answers
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I think that global DTMs should be used for flood modelling and mapping with caution. If you have no other topographic data available then they're better than nothing. However, ideally high resolution LIDAR or other surveyed topographic data should be used. Not only will these sources of topographic data generally have a better spatial resolution than global DTMs but they should also have a better vertical and horizontal accuracy as well.
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I am looking to purchase a very fast X-ray shutter if possible. I would like to keep the costs reasonably low. Does anyone know of any manufacturers that have a shutter below $1k or somewhere in that ballpark?
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the term 'very fast' is a contradiction to your wish of 'below $1k'.
In my active x-ray times I havn't seen any fast x-ray shutter below $1k. They are all about some $k.
You should for example specify:
a) open & closing time; min. open time(s)
b) x-ray beam diameter
c) x-ray photon energy or energy range
d) degree of attenuation
e) environmental consition ( e.g. in vaccum or at elevated temperatures, etc.)
Various types of x-ray shutters can be seen in the following link (simple google search result).
But sorry; price informations are very rare. In most cases one has to ask for an offer.
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Hi,
I tried the following command:
gmx trjcat -f *.pdb -o testfile.xtc -cat
and always get the following error: Fatal error:
gmx trjcat can only handle binary trajectory formats (trr, xtc, tng)
I checked the documentation several times but don’t find a reason for this error. Does anyone can help me?
Many thanks in advanced!
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I was trying to do the same thing when I found this question. GROMACS gives the error as it doesn't expect a list of pdb files as input, but a pdb file containing multiple frames. From the online manual:
"The pdb format can also be used as a trajectory format: several structures, separated by ENDMDL, can be read from or written to one file."
In order to convert the pdbs to an xtc file you first have to combine them into a single pdb file using something like:
for f in *.pdb; do cat $f >> traj.pdb; echo "ENDMDL" >> traj.pdb; done
Then use traj.pdb as the input to the gromacs file.
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Positively charged/cationic lipids are widely used as drug vehicles by taking advantage of the non-specific electrostatic binding to the negative surfaces of cells.
But if cells bothered to make negatively charged lipids, why don't they produce positive lipids?
Is there a known or hypothetical reason for this? Is it because it would disturb cellular function, or any other reason?
I would largely appreciate any comment.
Rafael
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In eukaryotic plasma membranes, you have negatively charged lipids majorly at the inner leaflet. Then the cell produces membrane proteins, which need to orient in the plasma membrane. They are oriented with the positive charge towards the inner leaflet... So here, the negative charge of the membrane lipids helps to orient the membrane peptides and proteins.
I believe the charge distribution throughout the cell is a complex thing, where some positively charged molecules help to guide the incorporation/trafficking/orientation of negatively charged molecules and vice versa. It evolved in this way. Sure, it would work the same if the lipids were positive and the peptides had an excess of the negative charge in the inner leaflet of the plasma membrane. This would be an alternative evolution pathway.
My comment is strictly my hypothesis and understanding of the topic. I do not have it backed by any study.
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Dear connections, I am looking for the possible causes of death of microorganisms as the case study below:
In the morning, when the lab engineer check microorganisms under a microscope, he observed that all the microorganisms in the bioreactor died out. It was normal yesterday, what could be the reason?
Thanks in advance.
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More options: virus, age, etc.
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Botrytis cinerea infection leads to the formation of necrotrophic lesions on plant leaves. In some cases (infection with several B. cinerea strains), these lesions are surrounded by the area of intact tissue with degraded chlorophyll (yellow colored). Is there any information about the presence/absence of the pathogen or pathogen-produced toxins in this “yellow area”?
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You can take a look at the research: Botcinic acid biosynthesis in Botrytis cinerea relies on a subtelomeric gene cluster surrounded by relics of transposons and is regulated by the Zn2Cys6 transcription factor BcBoa13.
By: Porquier, A. et al.
Also, you can see: Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea.
By: Pinedo, C. et al.
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when Pd supported on SBA-15 and the particle size inconsistence what will be possible reason in above mention reasons. Need answer with the reference.
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To clarify Alan F Rawle's answer:
Particle Size - the physical size of the particle
Crystallite Size - size of a single domain with the same orientation
Therefore, particles typically consist of multiple crystallites.
You are inherently measuring the different definitions above by using different techniques, and therefore MUST differentiate between them. TEM, specifically STEM (s is for scanning), can get you overall particle size. Diffraction within the TEM can give you lattice parameters and in turn the unit cell, however, this is fairly time consuming, complicated, and out of the scope of your question. XRD, by whichever analysis technique (Scherrer, integral breadth, Rietveld, etc.), you are measuring the scattering domain size, i.e. crystallite size.
The condition when particle size = crystallite size is when the particles are single crystals. In my personal experience, this condition rarely occurs. I've measured multiple nanoparticle systems on substrates and compared with XRD, and "agreeable" values are normally 10-30nm different. The reason for this deviation is the inherent broadening of nanoscaled materials in the XRD pattern. Also, in any type of microscopy, there is a limited sample size of particles, therefore counting statistics are poor.
Hope this clears some concepts up!
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I have designed a few pairs of sgRNA oligos using both CHOPCHOP and CRISPOR. I checked for their alignments within themselves. However, when I did the Primer-BLAST using both oligos (excluding the flanking sticky sequences). Only 1 of the 4 oligo pairs showed the gene of interest without any mismatch. All other pairs showed mismatch with non-specific genes, ironically these were ranked in the top in the sgRNA tool result. What may be the reason for mismatches and non-specific alignment? Please suggest.
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Ok. Mouse and human both.
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I used codon optimized Plasmodium falciparum apicoplast helicase gene inserted into pet28a vector system (from Genscript) transformed into Rosetta 2DE3. I did transformation for 3-4 times 1-2 times I got one colony and another time I did not get any colony. What could be the reason ? I also checked my competent cell and I got result. Even When I transformed this plasmid inserted gene into DH5 alpha , I got good colony and from that I prep.plasmid and then did transformation into Rosetta2 DE3 that time I got good result. But directly from stock into Rosetta2DE3 I could not get anything why ?
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Thanks a lot sir. I will definitely try it.
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I'm purifying a protein using bacterial expression system. Purification is done using affinity chromatography. While on the SDS PAGE it shows band at expected mass (17 kDa) but molecular mass obtained through mass spectroscopy is higher by 1kDa (18 kDa) using the Sinapic acid as matrix. Other protein with same molecular weight are giving exact mass in Mass spectroscopy.
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SDS accuracy could be the problem. It could also be due to extraction methods or the matrix itself causing breakages in the protein. Have you checked the lower masses for a peptide(s) roughly that mass change?
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The field energy density /gravitational energy density is missing in General relativity but in Newtonian gravitation, it is present and negative as expected.
As stated by Penrose not very accurately, about potential energy
"Although there is no room for such a thing in the energy–momentum tensor T, it is clear that there are situations where a ‘disembodied’ gravitational energy is actually playing a physical role.
Imagine two massive bodies (planets, say). If they are close together (and we can suppose that they are instantaneously at rest relative to each other), then there will be a (negative) gravitational potential energy contribution which makes the total energy, and therefore the total mass, smaller than it would be if they are far apart.  Ignoring much tinier energy effects, such as distortions of each body’s shape due to the gravitational tidal field of the other, we see that the total contributions from the actual energy–momentum tensor T will be the same whether the two bodies are close together or far apart.
Yet, the total mass/energy will differ in the two cases, and this difference would be attributed to the energy in the gravitational field itself (in fact a negative contribution, that is more sizeable when the bodies are close than when they are far apart)."
As a matter of fact what is negative is the binding energy which is localizable... what is not localizable is the potential energy.
There is substantial a difference between gravitational energy which is negative in Newtonian Gravitation and is a sort of BINDING ENERGY and Potential energy which is positive since it is "given" to the system of attracting masses.
It is undisputed that there is no room at all for a potential energy density in gravitation since it is not determinable from where such energy comes from, although it exists...it cannot be part of the "gravitational field"...
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<<Einstein's Lost Key: How We Overlooked the Best Idea of the 20th Century" ?>>
yes, Unzicker support the Variable speed of light and for me he is right....
a medium which acts with the VSL...
It is sure that in euclidean space the only way to explain the Shapiro delay is with a Speed of light which decreases by going across a gravitational field.
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A researcher should have a reasonable comprehension of the different sorts of research design to choose which model to carry out for a study. Like the research study itself, the plan of your examination can be comprehensively grouped into quantitative and qualitative..
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YES!!: Of course!
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How to controle fill thickness by using spray pyrolysis method? also give some reason, film thickness increases whil doping concentration increased.
In my case, The measured film thickness is 156, 316, 448, 623 and 841 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt. % of Cu-Y2O3 thin films. Please add the reason to cause this result.
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It is impossible to control the thickness in spray pyrolysis but you can reduce the error by rotating the substrates in order to get it more precise thickness for every layer . The sprayer rate must be constant and the time interval between two sprayer also must be constant
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the composite matrix material is preferred to conducting polymer rather than non conducting polymer?
what's the reason behind it?
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Hi everyone.
I am interested in studying the mechanical behavior of cells using nanoindentation experiments simulated by finite element analysis. Following the literature, cells usually show a viscoelastic behavior. For this reason, I would like to know what kind of viscoelastic model is more adequated for simulating the cells' response to indentation interaction.
Any comment or reference will be appreciated!
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Hi,
without having any idea about the specifics of cell modeling, a robust framework for viscoelasticity under large deformations is the one from this paper
I am also attaching some unpublished notes of mine with a simplified time integration.
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We cloned a viral gene into pET28a expression vector and expressed in E. coli. Then, expressed protein was examined by WB and other methods. However, unexpectedly, molecular weight (MW~35KD) of the expressed protein was found to be higher than its predicted (expected~30KD) MW. How could I explain it and what are the major reasons behind it?
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When a large earthquake occurs, some structures show great damage and some of them collapse, resulting in human casualties.
If you ask a civil engineer he will tell you that today's constructions are anti-seismic designed.
If after the earthquake some constructions collapse and we have victims, the civil engineer who did the study and the contractor who built the project have problems.
They accuse the civil engineer of incomplete static design, and the contractor of stealing the materials.
Is that so or is something else to blame?
In this article I will try to explain the unbalanced factors that can lie down and the most modern seismic construction, with the best seismic design and the best materials.
I do this for two reasons.
First to dispel the myth that there is an absolute anti-seismic design today.
Secondly, so that civil engineers and contractors are not unfairly accused of not doing their job well.
A rough construction may not suffer anything and the adjacent anti-seismic design may collapse.
How does this happen?
Unstable and uncontrollable seismic factors.
1) Coordination
(Coordination in physics is the phenomenon in which in a forced oscillation the frequency of the exciter is equal to the eigenfrequency of the oscillator, resulting in maximizing the amplitude.
Each oscillator can oscillate in a frequency range.
The instantaneous excitation of an oscillator is equivalent to the efficiency in oscillation of a certain amount of energy. This is free oscillation which occurs at a frequency that is identical to the oscillator's own frequency. When the oscillation is forced, its frequency is the frequency of the exciter. When the exciter frequency is the same as the oscillator frequency we have tuning.
Frequency is the number of repetitions of an event per unit time. Frequency characterizes any physical quantity that changes periodically, that is, it repeats the same values ​​at regular intervals. object)
During tuning the system has the maximum possible width and the maximum possible energy. If there are no damping forces, then the amplitude of the oscillation becomes theoretically infinite. Thus, the oscillation can become so intense that the oscillator is destroyed. If the energy supply is higher, then there is a risk of damage to the oscillator. The glasses have a specific eigenfrequency, which can be heard if we just tap them once. If we emit sound at this frequency, then the glass will oscillate at maximum width until the width becomes too large for the strength of the glass and the glass will break. )
So the frequency of the earthquake is unknown;
The first thing we do not know is the frequency of the earthquake because each earthquake has its own different frequency.
The frequency of the building is proportional to its height.
See in the experiment how structures with different heights react at different frequencies, to understand why even the best constructions are destroyed while the others that do not have an anti-seismic design may not suffer anything.
2) Duration.
A construction can withstand high acceleration for a short time or small acceleration for a long time.
If the acceleration of the ground is great and the earthquake has a long duration, no construction will remain upright.
3) Acceleration.
It naturally expresses (or describes) the rate of change of a body's velocity (ie how quickly it changes its velocity, at a random point in time).
Acceleration is basically the variable speed of movement (back and forth) of the structure.
The bigger it is, the more destructive it becomes.
The acceleration that will reach the bottom of the structure (and ultimately the one that measures the risk of destruction) does not depend solely on the magnitude of the earthquake, because the epicenter of the earthquake may be far away so the acceleration that will reach the bottom of the structure is very small.
The soil composition that mediates between the epicenter and the structure is another factor that increases or decreases the acceleration and oscillation amplitude.
The soil agitates the acceleration 2 to 4 times more than the rock.
The constructions are designed to withstand from 0.16g up to 0.36g depending on the danger of the area, and the importance of the project.
Of course they can withstand greater acceleration up to 0.7g if the earthquake does not last and there is no coordination.
The largest earthquake that has occurred in Greece was of the order of 1g
The largest earthquake in the world was of 2.9g and took place in Chile.
Even the maximum acceleration given by seismologists for each area which determines the hazard index of an area and on which the seismic design of structures depends, can be incorrect more than 10%
So the next time you see disasters do not swear that the civil engineer and the contractor are to blame.
Earthquake design is also a matter of cost.
Poor countries can not afford the cost of complete seismic planning.
Something cheap and safe can only come from the anti-seismic design I propose which transfers an extra external force onto the construction, in order to control inelastic deformation.
Today's seismic constructions have exhausted the dynamics they can offer for the following reason.
To become stronger they must increase the volume of concrete and reinforcement.
Increasing the volume of concrete and reinforcement also increases the inertial tensions, so after a point and then it is a free gift.
The external force transmitted by the mechanism of the invention on the structure coming from the ground, is a force without mass so the dynamics of the structure increases without increasing the inertia intensities.
+ many more.
Measured experiment with a natural earthquake acceleration of 2.41g on a scale specimen bearing my patent.
The essay did not suffer the slightest failure.
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We synthesized metal nanoparticles with using different reducing agents. I found that the smaller size and uniformity of metal nanoparticles by using NaBH4. I am trying to find out how the NaBH4 affects the size and uniformity as well as good catalytic properties of metal nanoparticles.
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Hi. I hope the below link might help you:
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Is there a specific reason why splenocytes are used to isolate immunecells from?
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Hello, Fa Ro!
Please You also look at the article:
THE CELLULAR INITIATORS AND EFFECTORS OF THE ADAPTIVE SPLENIC IMMUNE RESPONSE
In this section, cellular functions and organization specific to the spleen will be discussed. Most of the work identifying cell subsets, location, and function has been performed in mice, and how these compare to humans remains to be clarified.
Fig. 2 The cellular organization of the murine WP is dynamic.
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Kindly share name of companies that can do peptide analysis by LC-MS at a reasonable rate. Thanks
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Thanks
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It seems to be taken for granted that high fruit and vegetable consumption is good for you, what is the underlying observational evidence?
I am aware that vitamins exist, but it is hard to actually find vitamin deficiencies in developed countries.
It's not that I am skeptical of everything. There are many things I have been convinced of with a cursory look at the scientific literature; For example:
1. Vegetable consumption correlates positively with higher socioeconomic factors.
2. Higher socioeconomic factors correlate with better health outcomes.
3. Better health outcomes correlate with socioeconomic status regardless of access to health care (Whitehall)
4. Stress causes poor health
5. Lower socioeconomic cohorts likely have higher stress levels.
6. Debt is strongly correlated with heart attacks.
All of this suggests that Fruit and Vegetable consumption would correlate with good health regardless.
Every time I have brushed up against this question tangentially by accident, I have found evidence against Vegetable's status. When I wanted to know about fibre and mortality I found a large study that showed that whole grain fibre but not vegetable fibre consumption was correlated with lower mortality rates. It does seem reasonable to assume vegetable fibre consumption would be highly correlated with vegetable consumption. Another study on mice that looked at supplemental potassium found effects on blood pressure and mortality, and concluded that any benefit of vegetable consumption could simply be due to potassium that could easily be found elsewhere.
Now that I have turned my inquiry to this question more directly I am relatively unimpressed with what I can find.
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Fruits and vegetables contain important vitamins, minerals needed for our life. A diet high in fruit and vegetables can help protect you against cancer, diabetes and heart disease ,and others.
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I wanna know what is the reason and behind it.
The LigPlot+ and PLIP are designed in such a way that LigPlot+ shows more hydrophobic interaction while PLIP shows more hydrogen bonds, so that's the reason behind these two software.
But what about Discovery Studio? why it shows the lesser number of interaction? What is the reason behind it?
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Dear all,
I am currently doing CFA for WHOQOL-BREF measures that has 26 items, 24 items are grouped in 4 categories. factor 1 has 7 items, factor 2 has 6 items, factor 3 has 3 items and last factor has 8 items. item 3,4,26 were reversed coded and my sample size N = 250 after removing outliers(n=19), and found that the four factor structure did not satisfy the GOF index. Specifically, χ2 = 572.10 df = 246, the CFI = .902 and RMSEA= .071, p .001, CMIN/df < 3. AMOS wouldn't run SRMR on my laptop for some reason.
Ive followed guidelines and removed 4 items with loading less than .40, one at a time. and the final results were χ2 = 419.48 df = 202, p sig, the CFI = .932 and RMSEA= .064, p .001, CMIN/df < 3. MI were all < 15. In my opinion, I'm not seeing significant improvement, which is suggesting that four factor model is not a good model for my data. I continued doing CFA for the instrument as one factor model (as found in previous studies) and found similar borderline results.
Currently, i am not sure how to proceed and how to report the findings. And do i still report reliability for the four factor model (there were all satisfactory >.70 before and after removing 4items)?. Can i use the four factor model in my subsequent analyses, like convergent and discriminant validity (non healthy vs healthy group)
if someone could help direct me to a direction or share some input, i would be very grateful. thank you
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Please refer to my publication on scale development. It is mentioning EFA and CFA on the items extracted through the review of the literature.
Here is the link of the article.
Thanks
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I'm searching for crash requirements in transmission design. I know crash may impact transmission mounts, housing, differential and etc. is there any standard or formal requirement for this reason?
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I suspect the standards are for engines mounted longitudinally (rear-wheel-drive). In a crash the transmission could be the first large object pushed in the passenger compartment. However, with CAD we're able to create a crash-zone and (hopefully) direct the forces so the engine and everything behind it are pushed downwards. I'll suggest that our ideas for a protective crash zone now use the engine and transmission as stressed members.
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I am working on metamorphosed rocks and while conducting whole-rock geochemical analysis, the result turns out with a high concentration of (from 10 wt% to 32 wt% Al2O3 ) mainly in metabasalt where SiO2 concentration is 45 wt%-54 wt%. I have been searching for literature to know the reason why such variation happens, but couldn't get a reference that can give me detailed insight on it.
I appreciate it if anyone can emphasize to me the reason behind it and/or share with me any literature that can help me understand it.
Regards!
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Please check the effect of post metamorphic alteration or metasomatism in the rock if any.it can significantly effect the Al2O3 content of the rock.
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I am adapting a scale ( TIntS scale from Pretz, 2014 -23 items) into my native language. After I have done the following steps ( Gudmundsson, E. (2009). Guidelines for translating and adapting psychological instruments. Nordic Psychology, 61(2), 29-45.) I ran in SPSS a Factor Analysis - Principal Components ( Rotation - Direct Oblimin).
The problem is that 3 items from one subscale (Inferential Intuition) loaded on another subscale (Affective Intuition) (0.46; 0.56; 0.64 respectively) and not loaded on the Inferential scale (>0.40) -and I do not know how to explain that or why is that the case.
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Translation is a tricky thing, because you're dealing not just with different words for the same concepts but also with words that refer to subtly different concepts and the underlying cultural differences that intersect with language. So you should not be surprised to find that some aspects of a scale's structure change, especially if the linguistic/cultural groups are quite different from each other.
That said, it's worth checking the following. Did the original authors use the same statistical procedures as you did? As you know, different rotational procedures can lead to surprisingly large changes in the results. And what were the factor loading in the original study? It could be that the items in question were always a bit "muddy," with strong secondary loadings on the factor where you obtained the primary loadings.
Beyond that, you'll have to wait for someone with real statistical expertise to weigh in!
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Hi all
I'm new to marketing and consumer behaviour research. I would be grateful if you could share some classic theories in this field or any framework or theories you found useful.
In the past, I have used the Theory of Planned Behaviour (incl the theory of reasoned action), the Technology acceptance model, and the Unified theory of acceptance and use of technology.
It would be great to expand my (as well as those new to this field) knowledge to look at other models that marketing researchers have been using.
Your contribution is much appreciated!
Kind regards
Junxiong
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Junxiong Li Based on using BS policies when appropriate, use the insights and methods from social marketing as they engage with behavioural sciences to lead the practical convergence .
The novelty in research lies in 2 aspects:
1. Providing the comparison of the application of BS to policy with SM.
2.Employing the policy-making cycle framework to map the contributions.
You may want to consider sociology and communications theory?
Another promising area is policy-making and citizens’ sovereignty.
Good luck.
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I am a journalist and editor and independent scholar. I receive notifications from Research Gate of my published work. I have claimed them as mine -- which they are. Then I am asked to upload full text. The copyright belongs to those publications which published my work, so the full text is with them. Can I upload links to that work when it is open access. Otherwise for copyright reasons I will probably need their consent to upload full texts as published.
Helen Gavaghan
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Hi Helen, Uploading to ResearchGate would be considered Green Open access. Most reputable journals will have a policy on this which typically tells you which version of the article you can uplaod and how long the article will have been published for before you are able to do this. You can find information here SherpaROMEO [ https://v2.sherpa.ac.uk/romeo/ ] or check the journals website or simply ask the publisher. Who onws the copyright is genarlly assigned in the agreement you sign when your work is published. The advice is despite the demands of ResaerchGate, be clear about the copyright situation before you upload. I don't think anything really bad will happen, you might get a take down notice or somthing like that. Of course there are many advantages to open and free access to your work on a platform like ResaerchGate as well. BW Matt
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  • Why quantum dots are only semiconducting ? OR
  • only II-IV or III-V groups form quantum dots ?
  • can other nanoparticles form zero dimensional also ? OR
Why can't nanopaticles be quantum dots ? Why nanoparticles can be metallic, polymeric etc only. But when we talk about semiconducting nanoparticles, they are quantum dots ?
I am little bit confused that is it necessasry the quantum dots only formed semiconducting. Is it the definition of Q dots ?
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Quantum confinement effects appear when the size of the material becomes less than the Bohr radius of its charge carriers. In metals, the Bohr radius is 0.0529 nm for electrons. Therefore, the quantum confinement effects do not appear in metals and it is very hard to create a metallic "quantum dot". But, creating semiconductor quantum dots is more feasible because their exciton Bohr radius exceeds 3-4 nm.
I suggest you reading the chapters 3 and 4 of the following book. It explains the related subjects very simple:
Fundamentals and Applications of Nanophotonics, Edited by Joseph W. Haus, Woodhead Publishing Series in Electronic and Optical Materials: Number 85
Best regards
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I have gone through quite a few papers in the domain of computer vision, and what I found that pyramid pooling operations are often found to be superior over regular pooling operations. This has also been empirically shown in several papers. Although, I cannot find any proper reasoning behind that. Most of the papers use very primitive terms and reasons. Can someone please explain the reason in details. It would be really helpful if you could explain from scratch.
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agree with Aparna Sathya Murthy
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The proof from A. Wiles can be read here:
Dear Colleagues,
The request to conduct discussions strictly adhering to the declared topic of discussion without switching to a discussion of the personalities and education of the persons taking part in the discussions.
I could offer my own proof:
I am sending here to my own support a file with a "beautiful" triangle, which can be perfectly described simultaneously using the equations of the Pythagorean theorem and/or the equations of Fermat's Great Theorem, FLT.
Also, I know that Andrea Ossicini deals with similar issues. For example:
Of course, there are other attempts to provide evidence.
Or reasoning that is very close to the stated topic:
Of course, other similar links can be shown here.
It is interesting to know the opinions of SPECIALISTS and persons -AMATEURS-who are interested in this topic.
CONTINUED. Start was here:
Best Regards,
Sergey Klykov
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Dear Colleagues Drs. Faraed Salman Karl Pfeifer ,
My sincere thanks for your contributions here!
Of course, the proof from A. Wiles was the very first.
However, no one can deny that attempts to obtain simpler evidence have been going on for centuries and it is unlikely to stop this process. It would seem that one can agree that mathematicians no longer need to have something else in relation to this problem. Ie, as if there is no problem.
But, in my opinion, it is impossible to think in this way about all mathematicians in general.
C. McLarty, really wrote interesting articles:
comments:
I had commented about it in the "old" FLT-thread on May, 2, 2021.
Dr. C.McLarty had my preprint read in April, 2021, as I think. Please, look at the PDF (Attachment) of my page below on the right, approximately in the center, slightly below the number 264.
What can you say about "my" triangle?
I am also interested in finding someone who will analyze the work of Dr. Andrea Ossicini and other authors.
For some reason I cannot believe that this is not interesting.
Also, see the playful Attachment on intentional complexity in mathematics. Really, someone would deny the existence of such a problem?
Sincerely,
Sergey Klykov
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I've been studying stress/strain evolution in Si anode, and a few weeks ago, my half cell (Si/LiPF6/Li) started to behave abnormally, i.e. OCP drifts to higher value. The cell has three electrode setup with single crystalline Si (100) as WE, and LiPF6(1M in 1:1 EC/DEC) as electrolyte.
This issue started only a few weeks ago, before that the OCP of previous cells was stable. I already replaced electrodes, both Li and Si, and electrolyte with new ones from different batch, but I still saw the OCP drift. The glovebox for cell assembly has also been regened a couple of times and both H2O and O2 level are below 0.5ppm.
Now I'm running out of ideas, I don't know what could drive the OCP upward by ~0.25V. I also tested the equipment, with 22uF capacitor, charging effect was about 0.5mV, so charging doesn't seem so likely the reason. I attach the OCP, CV(0.01V to 1.4V at rate 5mV/s) and EIS data here. The CV data also looked very strange to me, the current is always negative during cycling.
I appreciate any input on this.
Thanks much!
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could you (re)check, please, if the wafer's origin of your WE, Si (100)[1,2], has been changed.
Maybe, initially, it was given a n-type and later, now, it is (replaced by a) p-type[3] (?).
1. How to Determine a Silicon Wafer's Orientation https://www.universitywafer.com/silicon-orientation.html
3. Si Wafer | Difference Between N-Type and P-Type Semiconductors https://www.waferworld.com/si-wafer-difference-semiconductors/
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why and when surirella diatom abundance increases in estuary and marine water and whar is reason of high abundance of surirella sp.? and
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I think there is not a single reason for a high development of Surirella in your estuary. Surirella is motile and commonly epipelic and therefore you easily find it in the estuary because there is a lot of mud. If you observe a big increase in its population, you will unlikely get to know why Surirella and not other epipelic diatom such as Navicula o Nitzschia grows. These might perfectly have grown instead. Moreover, keep in mind that you are talking about a whole genus which comprises many marine and freshwater species.
I think it is better to think in terms of algae biomass and/or groups. For example: Why do I observe a growing in planktic algae / benthic algae / cyanobakteria / green algae...? In your case, I do not know you study area and its problems and pollution sources, but I would probably ask myself why did I find an increase in benthic diatoms, for example, supposing you observed it. If you have just seen a switch from Navicula to Surirella, for example, it may have been just part of the annual sucession in your ecosystem and may not have any easy-to-find reason (and the actual reason may not be important at all in terms of monitoring or assesment).
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Recently, I noticed most of the researchers are taking support of third party persons and willing to publish their papers in a fast paid publication which are mostly fake and cloned and they wasted a lot of money. So, why researchers are not going for non-paid quality journals.
Is the time process taken by editors the main reason for it or any other reasons? Kindly, throw some light on this matter. Thanks.
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Publication and ranking has become a rat race. Scholars are increasing their publication without making any scientific contribution (some willingly and some under-pressure).
Institutions too encourage scholars/teachers to publish to increase their rankings.
The recent case of p-ranking and the craze among scholars is alarming.
and yes, non-paid journals take time and the fear of lagging behind pushes scholars towards paid journals.
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What is the reason for the poor performance of the nitrogen-doped porous carbon-supported cobalt single atom material in ORR, OER, and HER?
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Dear Ming-Jun, many thanks for asking this very interesting technical question. Of course it is very difficult to judge as an outsider what possibly went wrong in your case. My personal suggestion would be to discuss this problem in detailwith your PhD supervisor. Moreover, you can try to reproduce a procedure described in the literature and see if you get the same results. Unfortunately, research articles in this field do not report negative results and do not explain what could perhaps go wrong. When you have a look at relevant articles on this topic you only read about "Efficient.." or "Highly efficient...". For example, please have a closer look at the following potentially useful articles:
Cobalt single atoms anchored on nitrogen-doped porous carbon as an efficient catalyst for oxidation of silanes
High efficiency nitrogen doping and single atom cobalt anchoring via supermolecules for oxygen reduction electrocatalysis
Co, N co-doped porous carbons as high-performance oxygen reduction electrocatalysts
Single-Atom Catalysts for Electrochemical Hydrogen Evolution Reaction: Recent Advances and Future Perspectives
The latter article is freely available as public full text on RG.
Good luck with your work!
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In the 19 hundreds, many though that an explicit formula for the partition function was never going to be found. In 2011, finally, an explicit formula for the partition function was discovered.
For that reason, I am fascinated by how close do mathematicians think we are currently to discovering an explicit formula for prime numbers.
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The vitech result confirmed the strain which was s.pneumonia as well.Could anyone tell me the reason or suggest a possible mechanism?although the optochin disc that I used was from different origin.
Your help is very much appreciated
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One of the most important reasons of antibiotic resistance:
1. your strains is mutated S. pneumonia.
2. Your strain has received a copy of conjugated plasmid carrying resistance to optochin.
3. Your VITEK system report is false.
4. Your Optochin antibiotic disc is expired.
Try to diagnose your isolate by PCR then by sequencing. Perhaps your streptococcus belong to another streptococcal Lancefield group.
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I am doing pyrolysis of ZIF-8 and during pyrolysis there is a stay time at 350 degrees and then there is stay time at final temperature. I want to know that what is the reason for that stay time?
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Muhammad Umair staged pyrolysis is very weel optimized process of slow pyrolysis the low temperature but need lonfg yime in days for wood , the fast pyrolysis the high temperatures at lower time , but the combination this too first the voltaile hemicellulose as well the cabohydrate linked to lignin can me made to be seperated very fast even at low temperatures as very good products mostly hydrocarbonates leaving celulose and lignin de cabonations with hydrogen rich solow pyrolysis gas , the cjharcoal left is again decabonazied via fast pyrolysis to char m tar nancarbons , Thus the complete convversion is achieved in shortest time as well as better better products analysis made possible , other wise just one may get c02 ,co , h20 prolonged fast pyrolysis , incomplete partcial oxidations too
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Recently in India many people recovering from COVID-19 have of late been afflicted by black fungus or mucormycosis disease. What can be reason? how to diagnosis in early stage?
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Please have a look at this useful RG link.
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What are all the potential reasons for "having a Pathogenic genetic variant in an apparently healthy Individual without showing any relevant/linked clinical features?"
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Studies indicate that the penetration of pathogenic variants may be lower in the general population compared to target patients with a strong family history of certain diseases. It may be that people who do not have a strong family history of certain conditions may show incomplete penetration of pathogenic variants. In general, a lot of work needed to better understand genotype-phenotype associations and the penetrance of genetic variants. Kindly check the following RG links:
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Dear Colleague,
Part of my Ph.D. thesis needs to be completed questionnaire, which, unfortunately, due to Covid 19, we cannot attend the company under review. For this reason, I request supply chain experts who wish to complete the questionnaire to notify me, that I will email the questionnaire to them. It would be your generosity to respond to the questionnaires and also distribute them among your colleagues, students, and networks.
Thank you in advance for your help and cooperation.
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I have received comment'reviwer about
"In Figure 3, the authors correlated e4 and e20, e4 and e13, e20 and e13, etc. It is strongly necessary to explain the reason why those error terms need to be correlated in theory"
Could you someone give me an ideas to explain that ? See attached
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You must have a strong theoretical reason in order to justify, why some error terms should be correlated. For example, you may argue that error terms should be correlated because the underlying variables are under influence of a factor different from the latent dimension supposed to be measured, such as social desirability or some other response bias. In the article, you must explain the reason for error correlation.
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This Defect has seen nearly 2 or 3 plant in 1 hector of land.
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A large number of mutations affect sex determination in maize. Mutations that feminize the plants include the tassel seeds, which are characterized by the development of pistils in florets on tassel.
Such a mutation may have pleiotropic effects, causing the absence of outer covering of tassel ear.
Best wishes, AKC
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Hi everyone
I am working on a shell and helically coiled tube heat exchanger with laminar flow through the shell and turbulent flow through the coil tube. I have performed iterations for coil by selecting different types of turbulence models but in each case, energy starts to diverge after some iterations (images attached).
What is the possible reason for this?
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Attached is the mesh quality.
Mesh Quality:
Minimum Orthogonal Quality = 2.19097e-01 cell 8514 on zone 10 (ID: 392047 on partition: 0) at location ( 3.27922e-02 -4.20263e-01 2.32134e-05)
(To improve Orthogonal quality , use "Inverse Orthogonal Quality" in Fluent Meshing, where Inverse Orthogonal Quality = 1 - Orthogonal Quality)
Maximum Aspect Ratio = 1.23416e+01 cell 542 on zone 9 (ID: 580582 on partition: 0) at location ( 3.28492e-02 -5.49638e-01 7.91715e-03)
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I would like to know what is your favorite book about "the story of psychology" and the reasons why you love it! Thank you in advance.
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Here, as in many other questions on various topics (which is the best film, the best opera, the best novel, etc.) it is not possible to offer a single option or alternative, so I opt for five (5), among others and presenting them NOT IN ORDER OF IMPORTANCE:
- "HISTORIA DE LA PSICOLOGÍA", by H. CARPINTERO CAPELL and A. GREGO DÍAZ. CEF editions
- "Introduction To The History Of Psychology" by B. R. Hergenhahn. Ed. CENGAGE
- "HISTORY OF PSYCHOLOGY", by J.M. Burgos. Ed. Albatros.
- "HISTORY OF PSYCHOLOGY" by Thomas. H. Lehaey. Ed. Pearson.
- "HISTORY OF PSYCHOLOGY: SYSTEMS, MOVEMENTS AND SCHOOLS", by A. SANCHEZ-BARRANCO RUIZ. Ed. Pyramid
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Hello everyone
I get LPS/IL4-induced BMDM from mice, which inject some drug I want test. analysis the M1 (CD86,TNFa, inos) and M2 (CD206,Arg, Fizz1)marker gene expression by qPCR.
compare with PBS group, both of M1 M2 marker expression are decreased in my experiment.
tha same results find on flow cytometry.
I don't know how to explain this
Is this reasonable?
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Try to compare iNos/CD206 ratio between your experimental groups
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Dear Fellow Researcher,
I am trying to compile former research and records of Indonesian Architecture into a book. It will be too ambitious for me to collect all the tribes with distinct architecture. However, the progress has gone a long way so there's no reason to stop. We required some records, preferably which includes description or pictures of the vernacular building in Irian and Maluku prior to 1900s. Any contribution will be mentioned in acknowledgement. I will appreciate all the help. Thank you.
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More pictures
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I have a problem with electrochemical measurements of RGO. The Cv curves have eight-shape instead of rectengular in 20mV/s. However, in 50mV/s the curves are quite good. I do not know what the reason is. The sapes are attached:
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Thank you so much for your answers and interest. I will try again.
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For nanocomposites LDH-based, What is the reason for the open loop in N2-Adsorption/Desorption isotherm?
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Same issues as before. Use the filler, check life-time of LN2 in your bath, add more sample. Additionally, impo, 5 sec equilibration for gemeni method is too short.
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While working on my unsupervised learning project, I had found that the widely used Davies-Bouldin (DB) and Calinski-Harabasz (CH) Indexes are not working. While finding the reason behind it, I had found that this is because the data I am using is sparse. Are there any other clustering evaluation methods (index or metric) available that work for sparse data?
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In order to analyze sparse data, the best way is to perform dimension reduction, principal component analysis or singular values decomposition for data shrinking or removing the unwanted features exists in the given data base.
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In cyclic voltammetry, what is the charge (positive or negative) on working electrode at anodic peak. What is the charge (positive or negative) on the working electrode when negative and positive potential is applied? Should be the anodic peak always in positive potential and cathodic peak in negative potential? Oxidation peak is anodic or cathodic? I am getting both the peaks in positive potential, is it ok? what is the reason?
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It is a bit complicated, but the simple answer to your question is, yes, you can get both the anodic and cathodic peaks in the same potential range. It all depends on the standard (formal) potential of the particular redox couple you are studying. The easiest way to remember the difference between the two types of peaks is that, if you are scanning in a positive direction, that is, from more negative to more positive potentials (or less positive to more positive or more negative to less negative potentials when you are in the same potential range), the CV wave you observe is the anodic (oxidation) peak and vice versa for the cathodic peak. It may seem complicated, but once you learn about CV, you'll be able to understand a lot about the redox couple you are studying. It is a very powerful analytical technique.
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Dear all,
I am working with a transcriptomic technique that I need to permeabilize the cell to release and collect the RNAs. I am using 0.1% pepsin for 40 minutes. However, this is working very well with normal tissues (except skin) but it did not work with the solid tumour tissues which have the dense nuclei. Anybody knows the reason? Should I increase the concentration of pepsin?
Thank you so much.
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Thank you Lo Pham for elaborating. I have experience with mammalian brain tissue RNA extraction. I took no more than 30mg of tissue and used Qiagen Lipid tissue kit for RNA extraction where homogenization is performed in Trizol (Qiazol) using a polytron/tissue homogenizer followed by chloroform for phase separation (Upper aqueous phase whre RNA is) then purification by column filtration. The RNA yield was substantial. If you want to also collect protein before doing RNA extraction then retain the organic pink phase where the proteins would be, but first eliminate the interphase where DNA is by ethanol precipitation adding ethanol at a 1/3 of the trizol volume you used. Then vortex or mix by pipetting, let it sit for 3 minute then centrifuge at 2000xg at 4degrees for 5 mins. The supernatant is where your proteins are.
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There is a lot of research on AI-based air pollution forecasting, but very few have put up a reasonable explanation in this regard.
I want to know what might be the reasons for the performance drop ??
Is it a problem of data length or any other issue ??
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It's very simple. All forecasting methods are based on the search for patterns in the retrospective data and on the assumption (hypothesis) that these patterns will be valid in the future for the forecast period. In other words, it is assumed that the training sample is representative for a certain period in the future. This period is called the period of ergodicity. But this is an incorrect assumption. Sometimes the patterns in the modeled domain change. The period of ergodicity is violated. New patterns are being formed, although the old ones may remain. Therefore, the point of violation of ergodicity is called the bifurcation point. It is necessary to predict not only based on the patterns of the past period, but also to predict the risks of violating these patterns. I did it back in 1994: http://lc.kubagro.ru/aidos/aidos02/7.4.htm (see Figure 7.2).
Это очень просто. Все методы прогнозирования основаны на поиске закономерностей в ретроспективных данных и на предположении (гипотезе), что эти закономерности будут действовать и в будущем на период прогнозирования. Иначе говоря, предполагается, что обучающая выборка репрезентативна на определенный период в будущее. Этот период называется периодом эргодичности. Но это неверное предположение. Иногда закономерности в моделируемой предметной области меняются. Период эргодичности нарушается. Формируются новые закономерности, хотя могут оставаться и прежние. Поэтому точка нарушения эргодичности называется точка бифуркации. Надо прогнозировать не только основываясь на закономерностях прошлого периода, но и прогнозировать риски нарушения этих закономерностей. Я это делал еще в 1994 году: http://lc.kubagro.ru/aidos/aidos02/7.4.htm (см. рис. 7.2).
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I have prepared cerium oxide nanomaterials. I obtained the spindle-shaped morphology by agglomeration of a number of nanorods. What chemistry can be involved in the agglomeration of nanorods into spindle-shaped morphology.
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Dear Surjeet Chahal many thanks for your very interesting technical question. Spindle-shaped cerium oxide nanomaterials have been observed by other researchers before. In this context please have a look at the following potentially useful articles:
Facile Fabrication of a Cerium Oxide Nanorod
This paper is freely available as public full text on RG.
Construction of spindle structured CeO2 modified with rod-like attapulgite as a high-performance photocatalyst for CO2 reduction
Fabrication and Application of CeO2 Nanostructure with Different
Morphologies: A Review
(see attached pdf file)
Nucleation and Growth of such cerium oxide nanomaterials have been outlined in detail in the following relevant article:
Synthesis and Characterization of 1D Ceria Nanomaterials for CO Oxidation and Steam Reforming of Methanol
Good luck with your research and best wishes! 👍
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I am receiving two bands in the methylation pcr rxn and single band in unmethylated rxn of VEGF A gene promoter what would be its reason.
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IN MSP, one may get bands in Methylated sp and Unmethylated sp primers, which shows hetrozygous nature of methylation status. if you are getting 2 bands in a reaction, suggestions are below
1. Try to minimize primer concentration. or
2. Increase 1-2 deg of annealing temperature.
and always use control as said below
3. An individual PCR reaction specific for methylated and unmethylated primers may be set without adding template.
4. Use hotstart PCR.
good luck.
Dr.R.Suresh kr.
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Texturing the surface of silicon wafers for photovoltaic applications using alkaline solutions gives pyramids of different heights . What is the reason for this? and are pyramids of great heights the best or random?
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Dear Abdallah,
I remember I read somewhere that the optimal pyramids' height should turn around 5 micrometers.
Best wishes.
Lyes BENHARRAT.
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I have observed a cloudiness/film formation (image attached) on the surface of wine after the anaerobic fermentation. Anyone can explain the reason and how am i going to counteract this issue?
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Once on your glasses, this cloudiness is hard to remove. You could try soaking the glasses in vinegar to dissolve the minerals, or rub the affected areas gently with bicarbonate of soda or nail polish remover, and then washing and drying by hand. I've also heard effervescent denture cleaners can help! https://www.decanter.com/learn/cloudy-wine-glasses-ask-decanter-384113/
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Whether the length of a thesis is a mark of quality is a subject that is treated differently in different fields and universities. While it is universally accepted, in some fields e.g., the arts and ecology, that a well-researched PhD thesis ought to achieve some number of words, some fielded e.g., mathematics and physics, may not place a lot of emphasis on the length of PhD theses. What is your opinion of this subject, and what is the practice in your field/ institution.
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The university might have requirements. Your advisor should let you know this and your university should have its requirements for the papet available to you on request.
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Some journal' editor said that "the authors should provide proof that the paper has been proofread by a native English speaker". Is this a reasonable request?; if yes, How can I provide it.
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Well narrated @ Wolfgang R. Dick
I agree with you
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In my simulation, i have used Cu2Te as an absorber layer.When i add Bsf layer effeciency get decreased.
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When a BSF layer is added,
The fill factor and overall current density decrease leading to a decrease in the overall efficiency.
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Dear all,
I'm doing some study on speech acts, and I'd like your help figuring out how to say congratulations in many ways. I'd appreciate it if you could inform me if this is the case. Please state your country as well as the reason for your contratulations.
Regards,
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"What are different ways to say congratulations?" --- Luqman M Rababah ask.
In my country, Portugal, when we want to says congratulations, we used the term "parebéns". This term tends to be used when we want congratulate someone for something worthwhile s/he achieved or attained. For example, we say "congratulations" to a student when s/he gets good or excellent marks in an exam situation.
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Hi all, I ran into a problem with the active power filter. When the modulation ratio goes above 1, it goes down to better levels. What could be the reason? Shouldn't the modulation ratio be m <1 in such applications?
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Thanks for recommending the study, but in such applications, the modulation ratio is required to be ma <1. I don't fully understand this issue either. Why do we get better thd levels when ma>1.
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A typical wheastone bridge consists of 4 resistor is amplified by a instrumentation amplifier and an ADC reads the output value. It is required to work under the exposure of UHF signal (900MHz band), the Tx power of the UHF signal is 1W. If the UHF signal is not presented, the ADC value is stable. However, if the UHF signal is presented, the ADC value will fluctuate. For example, under a fixed gain, the ADC value is stable at 20 but it will ripple between 20-400.
The reason is that the wires(traces) on the PCB act as antenna which rectify the RF interference. As a result, a rectified voltage will be added onto the input of INA (Instrumentation amplifier).
To eliminate the rectified voltage, a low pass filter (LFP) [1] as shown in the figure is added at the two input port (V+, V-) of the INA. The LFP indeed works and it reduce large amount of the rectified voltage. However, it is not enough. The ADC value will still ripple between 20-100. Also trying to change the capacitance of the filter capacitor but it does not help.
Any solution to tackle this remain voltage ripple?
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Application note of the ADC usually presents solutions for such problems.
It seems that the solution must be focused mainly on the PCB design (the power layer, the ground layer, vias to ground layers, filtering capacitors....).
However, using BALUNSs (balance to unbalance transformers) at the input of ADC is very effective.
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I have a white organic powder. When I dissolved it in DMF, a colorless transparent solution was observed, but when I dissolved it in THF, I obtained a yellow-brown transparent solution. I tried to remove THF and got brown powder. NMR indicated no difference. What is the reason for this phenomenon?
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It depends on your solid-state powder and the interaction between it and the solvents... In general, I think this article will give a good idea for this phenomenon.
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I got XRD peak of pure CNF paper at 10 degree and another one at 25 degree. I have references of 25 degree but not for 10 degree. can anyone suggest what could be the possible reason behind XRD peak at 10 degree of pure CNF?
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Most of the literature used partial cytochrome b gene sequences for animal phylogenetic studies instead of the complete sequence. What is the reason behind this where full or complete cytochrome b gene sequence could explain more details?
Thank you very much!
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Hi!
You got the answer.
Simply to show/prove populations are phylogenetically distant from other species three regions of mtDNA viz. cytochrome b, 16S RNA and and D-loop regions will be used.
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In the literature we can find research on the relationships: (1) emotional state and disease; (2) nutrition and disease. Can the two factors be considered together?
For example, if a person watches TV while eating - could this be a reason to get sick in the future?