Questions related to Microbiology
I am working on the strain Penicillium chrysogenum, but I have a problem with cultivation in a liquid medium and it doesn't grow or its growth is not properly. As we have a general incubator (33c and 162rpm) I use this incubator for the project. we have also an incubator that has not shaker but I can change its temperature. I prepare the culture medium that is attached under this question and I pour it into a flask and autoclave it at 121 c (all of the materials in one flask). I use solid culture as inoculum (one loop). 33c and 162rpm.
What are the names of strains of Saccharomyces Cerevisiae which are generally used in production of nutritional yeast?
I would like to know how I can get a standardized suspension of Aspergillus brasiliensis. I used a paper Whatman No. 4 to separate hyphae and spores when I check in plate count at various dilutions get inconsistent results after incubating at 20 ° C to 25 ° C in three days.
Question 1: For example, if 70 bps of complete homology exist between a fragment and host chromosome (in let's say E. coli), will all 70 bp's be exchanged between the two DNA molecules?
Question 2: Will each strand go through recombination as in holiday junction etc...?
Anybody knows about Pride conference, does it is authentic, please let me know because I have received invitation as one of the speaker in the conference. Details regarding conference and contact person are given bellow,
“International Conference on Microbiology & Infectious Diseases" which is scheduled at Hilton Rome Airport , Rome, Italy from November 08 - 10,2021.
Program Manager | Microbiology & Infectious Diseases
4 Lombard Street, London, EC3V 9HD, UK
T: +44 - 2392160593
Do you think the COVID-19 pandemic will significantly impact on water systems? With respect to recreational, surface and groundwater quality and supply?
I want strong, constitutive expression of my protein in m. smegmatis.
I have a strong, constitutive promoter. However, the RBS is very weak.
I want to exchange the RBS for a new one that is better.
However, I worry that I will "break" the promoter when I try to add the new RBS.
How do I determine the important parts of the promoter, so I can replace the RBS without destroying the promoter?
can I use the spectrophotometer to get the OD for my bacterial suspension before doing a disk diffusion antibacterial test to have a 0.5 McFarland turbidity , and if yes at what wavelength and what value should I get at said wavelength ?
I have listeria selective agar (oxford formulation) and listeria selective supplement.
I need to know what is the best diluent for enrichment of listeria.
is it possible to use Maximum recovery diluent or Buffered peptone water in enrichment step?
thanks in advance.
If I want to transport the sterilized laboratory glassware and bacterial culture in petridshes from one lab to another lab of a short distance then what will be the complete best strategy to avoid the contamination??
I am Performing the pomegranate juice evaluation, So Please help me with the process of microbiological assay of vitamin or provide the link of related paper or website
I and working on a project for extraterrestrial life, and i need few work on the titled topic. If is there any data, recommend it or please discuss the evaluation mechanism.
Muhammad Furqan Ali
We have isolated several strains of an organism from cultures of canine and human blood and subcutaneous nodules (using sterile techniques of course). These isolates have been subcultured on PDA and/or PD nutrient broth and found to be similar in appearance, both the macroscopic colonies, and the microscopic swabs viewed in wet-mount preparations. The organism stains positive with calcoflur white (contains chitin or cellulose) and seems capable of forming a variety of tertiary structures, depending on environmental conditions (ie, hyphae/pseudohyphae, sporocyst-like structures etc..). One universal property is that the small cells/spores etc... become motile within about 15 minutes after addition of sterile water or sterile saline to the smear. The “swimming” pattern appears eukaryotic rather than prokaryotic. I’ve attached a video showing time-lapse of about 25 minutes, during which a drop of stained liquid containing these possible “zoospores” undergoes several morphological changes. We think this represents the process of “zoospore induction” and eventual zoospore settling (maybe with release of stored mucilage etc... causing the white “fluid” appearance that develops upon the termination of “zoospore” motility?). We are awaiting WGS results on the cultured samples, and hopefully those sequencing studies will clarify the identity of these organisms, but in the meantime, we are trying to better understand the “behavior” we are seeing here, and figure out how it might relate to the apparent ability of this organism to infect and cause illness in, mammalian hosts. As far as we know, pythium and lagendium are the only zoospore-forming pathogens currently recognized as mammalian oomycete pathogens. We do not think this organism to be either of those, but we would appreciate help from anyone familiar with the movement patterns of zoospores from those (or other) pathogens to let us know if the movement seems similar to what would be seen in standardly done zoospore induction protocols and/or if there is an explanation for the “bleaching” effect that occurs after the motility ceases. Thank you!
I'm trialing a method of culturing the plant microbiome and I'd like to know if a majority of the microbial cells will still be viable for culturing if I store the whole plant sample in a -80 degree freezer. Will I only be selecting for and ultimately culturing micro-organisms that can withstand -80 degrees, or will the microbiome still be as viable for culturing had I processed the samples before freezing.
I need to do antimicrobial testing of a sample with gram-negative and gram-positive but I can only do BSL 1 in my lab. S.aureus is often used in the literature of similar experiments but it is BSL 2. What would be another gram-positive BSL 1 organism I can use instead?
We use malt extract agar with addition of 5 % tartaric acid to adjust the pH more acidic for yeast &mold counts (suppress the growth of the bacterial flora). The colonies formed in this medium were as in the image. What can we think about the white non-uniform growth in this medium? We would be very happy if you could share your experience.
I am trying to establish a growth curve for E.coli k-12 strain. I picked a colony from a fresh overnight plate and inoculated in 20 ml of fresh TSB in a 50 ml flask for 18 hours at 37 degrees Celsius at 220 rpm. Then I transferred 100 micro-liters of this culture into 250 ml of fresh TSB in a 500 ml flask at 37 degrees at 220 rpm. I took 5-ml aliquots immediately at time 0 and then every hour for up to 8 hours. I took optical density measurements of the aliquots and also did plate counts to get cfu/ml data. My calculated generation time was about 1 hour which seems to be a bit slow for E.coli and I was expecting to only get a lag phase of about a couple of hours. Does the outcome I obtained reasonable for E.coli? My plot has the typical shape of E.coli growth curve but the time I obtained for generation time and lag phase seem off to me.
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (
Thank you for your input.
Hello, I have a few hundred trimmed 16S metagenomic sequences from the Human Microbiome Project online database that I would like to search for the presence of several strains of bacteria via pairwise sequence similarity. Does anyone have any recommendations for software that can achieve this?
I am not a bioinformatician by any means, so something that has a user-friendly UI and can run on Windows would be much appreciated (although I can make my way around a command-line interface if need-be)!
As an aside, can I make a reasonable claim for species-level identity solely using 16S sequence identity? If so, is the >97% threshold generally accepted or is there another metric (e.g., score-based) that is more reliable?
I am currently researching the impact of different antimicrobials on the amount of endotoxin released from two different gram-negative bacteria types. I am using nutrient broth as the growth medium and it will end up being transferred into my samples for endotoxin analysis. I am using the ToxinSensor ™ Chromogenic LAL Endotoxin Assay Kit and a spectrophotometer for my analysis. The instructions state not to use a colored sample as it will interfere with the color change when detecting the presence of endotoxins. Is there a way to account for the color of the nutrient broth as the "standard" like I would for the water-blank step or do I need to remove the color completely? How could I remove the color without using something that will damage my sample?
If needed for reference, the instructions for the kit are listed at this link: https://www.genscript.com/product/documents?cat_no=L00350&catalogtype=Document-PROTOCOL
RNA vaccines follow a different strategy, without using any "real" component of the virus at all. Instead, researchers aim to trick the human body into producing a specific virus component on its own. Since only this specific component is built, no complete virus can assemble itself. Nevertheless, the immune system learns to recognize the non-human components and trigger a defense reaction. So May I ask, What are your opinions about the safety and efficacy of the BNT162b2 mRNA Covid-19 Vaccine?
I am preparing a metagenomics experiment from the soil sample. I will use Oxford Nanopore technology, which has only limited throughput (approx. 8 GB), so I need to discard all eukaryotic cells before the sequencing (because their genomes are really large, and would saturate the device quickly). So I am interested in the simple and reliable method, how to obtain only the prokaryotic and viral species. Is it a good idea to use some syringe filters? E.g. with the pore size 1 micron? So the eukaryotic cells will be stuck on the filter surface, and bacteria, archaea, and viruses will go through?
Thanks for all suggestions,
Hello everyone, I'm a biologist who is interested in the astrobiology and microbiology. I want to study master degree in microbiology and emphasize the thesis project in astrobiology. However I have never worked in something related with that, I lack of experience in that fields. On the other hand, a preview of the project is a requisite to apply for scholarships and study the postgraduate course in any university in the world.Recently I finished the latest book of microbiology of brock, also i have read books of astroiology, and papers, with them i got many ideas to research. However most of them require special devices which are able to simulate extraterrestrial enviroments such as venus, mars, etc. I'm living in Australia and apparently none of the universities in the country has this kind of machine. In addition to this, the development of one of this devices are too expensive and needs specialized personal to be constructed. So I had to read more about anothers possibilities and I got more ideas. But searching for papers, I noticed that always someone has already do the same (sometimes with differents kind of methods that I tought ). Am I being too exigent with me trying to do something unique, new and original? what can I do? it doesnt matter how much I try getting new ideas of research, always looks that someone already do the same. I'm stuck and out of ideas and time.
Thank you for read, I'll appreciate any advice.
I`ve been attempting to extract RNA from M. tb grown in lipid media using the Trizol method and the Direct-zol kit from Zymo Research. The growth of these strains in this media is minimal (reaches OD 0.6 - 0.9), so after extraction I`ve been getting low concentrations and low absorbance ratios for A260/230. The 260/280 ratios could also be better. Any idea on how I can improve these purities?
I streaked e.coli from the glycerol stock on an agar plate, and after 48 hrs, I only got three single colonies on the plate. The colonies look fine. However, since the only thing that grew on the plate is these three colonies, I'm a little worried about contamination.
This is my only stock, and I've been trying to revive the same bacteria and prepare some new glycerol stocks for a couple of weeks. But this is the only time that I managed to get results.
Do you think it's a good idea to use these colonies to make new stocks? Or should I continue trying until I get proper colonies on my plate?
Hi expert out there!
I'm using crystal violet staining to determine gram +ve and -ve bacteria, which is a very traditional method, but at the same time, I noticed that crystal violet also can use for cell viability tests. It seems that the sample that I'm going to use involves cells too, so my question is how can I minimize the chance for the cell to get stained but instead only the bacteria (ie increase the decolorization time will help?)?
Thanks in advance!
my name is Riccardo and I work as a microbiological analyst in a pharmaceutical company.
It's been a while since I started looking for alternatives to the huge amount of plastic we waste everyday with petri dishes, but I can't find anything.
I'm looking for alternatives both to stop using petri dishes or using biodegradable ones.
Glass is quite hard since we perform a lot of analyses and could be a complex solution.
Thanks everybody for anyone who reads this or gives some suggestion!
Hope everyone fine.
I'm having a doubt regarding that I'm going to do several in-vitro assays in subsequent days with the bacterial cells. In this regard, Shall I Culture a particular concentration of bacterial cells and store it in the refrigerator and reuse it? Will that be advisable? Will the live cells behave as normal as before?
Could you please share with us your latest scientific achievements (i.e. papers, books, etc) regarding anaerobic digestion technology? Your worthwhile findings would definitely respond to the need for "Engineers without borders" worldwide for tackling the energy crisis, especially in the Global South.
Plaese discuss about your achivements.
Dear Friends and Colleagues,
To date very few journals are available to accept video articles for submission/publication in our field, although these types of articles are of paramount importance in microbial ecology in order to share new techniques and approaches.
For this reason, I decided to open a Collection titled “Multi-targeted approaches to evaluate microbial interactions and ecosystem assemblages in diverse environments” on the Journal of Visualized Experiments (JoVE): https://www.jove.com/methods-collections/919.
The purpose of the Collection is to offer a comprehensive overview of wet- and dry-labs perspectives in the field of microbial ecology.
The most recent Impact Factor for JoVE (ISSN 1940-087X) is 1.163, according to the 2019 Journal Citation Reports released by Clarivate Analytics in 2020, and the Journal is currently indexed in the major databases, including PubMed, EMBASE, Scopus and Web of Science.
After submission, each manuscript will be editorially and peer reviewed, which is typically a 1-2 month process. Once a text article passes review, a script will be generated. Generally, a filming date will be scheduled within 4-8 weeks after acceptance. A videographer will be sent to the authors’ site to film the procedure.
If you are interested, either message me or submit an abstract here:(https://www.jove.com/methods-collections/submit-an-abstract?collection_id=919).
Prof. Elaine CP De Martinis, Ph.D – Full Professor, University of São Paulo, Brazil
Otávio GG Almeida, B.Sc. – Ph.D. candidate, University of São Paulo, Brazil
We have lost many flasks due to contamination to B. cepacia. We have added Pen-Strep to no avail.
What are other antibiotics that could be used in culture media that does not belong to b-lactam or aminoglycosides class of antibiotics?
I want to know about the different media used to check the growth and characterization of cadmium resistant bacteria to know the best media for its growth and also to aid identification of the bacterial species, what are the most commonly checked media for that?
We are also doing an experiment about siderophore. Can anyone please inform us why the color change occurs in the CAS media? I mean what chemical reaction is going on there?
Thank you very much in advance.
I tried to transform the pET32a plasmid in dh5 alpha cells a couple of times. The first time I got no colonies after spreading the bacteria on the ampicillin plate, and the second time I only got 3 single colonies.
I also transformed another plasmid which contained an ampicillin resistance gene in the same dh5 cells, to make sure my cells are competent, and got a plate full of colonies.
Is it normal to get only three single colonies after transformation?
Are these single colonies reliable enough to use for plasmid extraction?
I am performing wastewater treatment thought different microorganisms such as microalgae and afterwards determining the lipid content in the biomass though Bligh and dyer method. Due to the COVID restriction, we are allowed to have limited time in the laboratory and therefore, a lot of samples should be stored and tested later.
I have to store my sample for cell counting and lipid determination?
For cell counting, Can I store my sample in ethanol to persevere it and count it later?
(0.3 ml sample and 0.7 ml ethanol)
For lipid: Can I store my liquid sample at -20C. and perform the lipid determination by Bligh and Dyer method later?
The past few years have seen a lot of new cyanobacterial taxa being described using a polyphasic approach. It will be interesting to know that what are the various good things about using this approach and importantly, are there some particular taxonomic groups/clusters that are still unresolved where the polyphasic approach is still to give any proper answer?
What further developments do we anticipate in the coming years? What are the new techniques/methods that can be further incorporated for a better understanding of cyanobacterial taxonomy?
I am a new PhD student with very little microbiology experience and I have a few questions regarding the growth of Lactobacillus Reuteri which I hope you can help me with.
I want to grow L. Reuteri so that I can use it to create a standard curve for quantifying my bacterial qPCR data. I know lactobacilli are facultative anaerobes that grow best in anaerobic/microaerobic conditions. However, I do not have access to any anaerobic equipment in my lab.
I am planning to purchase the following Lactobacillus reuteri Kandler et al. (ATCC® 23272™) strain from ATCC which says it is aerobic on the product webpage so I am assuming I can grow it aerobically? (https://www.lgcstandards-atcc.org/products/all/23272.aspx#generalinformation).
1. Could you please provide me with a step-by-step protocol with the best way to grow this ATCC® 23272™ strain in the lab without anaeorobic equipment?
Since I am not interested in the fermentation products, I only need to make a standard curve so surely aerobic growth should be fine?
2. If not, could someone provide me with a step by step protocol for microaerobic growth without specialist equipment?
3. From your experience, which lactobacillus species grow best in oxygen?
I have a general idea on how to grow it but I am really struggling without access to any protocols or any Microbiologist contacts... The research papers I have found are too vague. I would really appreciate your advice.
I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups (treatments) and when I analyse the data on OmniLog I don't know when and how to normalise the raw data : before or after calculating the parameters ? I would like to know if there's a significant difference on the metabolisation of a substrate between different groups but I don't know how to procede with this package.
Would you have any advice on how to treat this kind of data ?
For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I have only ever grown E.coli before so I'm not confident with the lactobacillus growth conditions.
Can you please provide me with some information about growing different lactobacillus species? Preferably aerobically as it will be more difficult for me to access an anaerobic chamber...
I was thinking of ordering some cells from ATCC (https://www.lgcstandards-atcc.org/products/all/23272.aspx#generalinformation). Is it possible to use the same agar and LB broth recipe as I'd use for e.coli or does it need to be altered for lactobacilli?
Any advice would be much appreciated. (As detailed as possible).
That vaccines have been produced in such a short time, makes me doubt if they will help fight this pandemic and if they will not do us more harm.
Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each primer. I understand then that have to mix them equimolarly, am I right? Has anyone used them?
The coronavirus pandemic has brought the world to a grinding halt and no doubt has altered the lives of each one of us. I think COVID-19 also has an impact on our normal research (i.e. related to our relevant field) and diverted us to work on various aspects of coronavirus to help combat the virus.
What's your opinion in this regard?
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated from a soil sample, we assume the soil is acidic. Is this also applicable to the type of mineral microbes love? How would this microbes tell the presence of such mineral?
Your contribution would mean so much for me
I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.
How can I know the stationary phase of growth of several bacteria?
We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the stationary phase of a particular bacteria. Perhaps we have many bacteria and there is very limited literature on few bacterial stationary phase. Further the information is not available for many bacteria. Could someone suggest us any manual or a paper which describes stationary phase of these bacteria.
How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally?
I understand you could use specific primers for the 16s V region of a bacterial species e.g. Lactobacillus Reuteri but how would you then quantify the CT values generated from your sample?
For instance, would you include a standard curve of Lactobacillus reuteri on the plate? If so, how is this possible if you cannot culture the bacterial species?
Instead of culturing, is it possible to order in bacterial samples to use on a qPCR plate for quantification? e.g. Bacterioides?
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have turned red or pink.
Evaluation of the overlayed colonies was done after 1 h, 2 h, 3h and 6 h. I tried different TTC concentrations: 0,1%, 0,05%, 0,03% and 0,01%.
I have attached photos of the colonies which were affected by EthBr and which were not.
I read an article and actually met someone who suggested that human breast milk contains microorganisms and could be a source of novel bacteria
I am working on the production of exoploysacchardes from Psychrophilic bacteria. I need deproteinization of the sample before precipitating EPS. Any alternative method will be appreciated.
The common method for phosphorus solubilization efficiency measurement of soil microbes is zone formation in agar media (generally Pikovskaya media) but this is a qualitative study. However, there is also a liquid culture method with insoluble phosphorus in liquid media. The estimation of available phosphorus by ICP-OES is quite promising and precise.
I need to know effect of heat treatments that are made when making camel milk powder on chemical , microbiological properties and shelf life of camel milk powder compared to cow milk powder. Thanks
I received several strains of bacteria from another lab far away. However, one of the samples appears to have high fungal contamination. When I grow it in broth, I see lots of fungal contamination. It would be difficult and time consuming to have them send it again, and I worry a new sample would still be contaminated. I am confident it's not my reagents that are contaminated (uninoculated media showed no growth, and other strains they sent were fine).
Is there a good way to recover my bacteria from the fungus?
0.45 um filters? Addition of cyclohexamide?
I have a culture of heterogeneous bacteria that will NOT form endospores.
This bacteria was originally isolated in nature and is a complex culture of multiple species.
I want to be able to preserve samples of these cultures for future study, but I cannot get them to last more than a few weeks in a refrigerator.
I CANNOT freeze this bacteria.
Is there a way I can put this bacteria in a quiescent state that will preserve it for a few months in a refrigerator?
I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.
I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom.
The protocol worked well and I thought I got my desired knockout strain. However, I have now found that I cannot electroporate plasmids into my new knockout strain successfully.
I don't think it's something unique about the gene I deleted, as I have another strain with the same gene interrupted by a transposon and it's fine (electroporations normal).
I've tried electroporations repeatedly with new reagents and another strain as a positive control. Every time, my old strains work (I get lots of electroporated colonies), but my new knockout strain gives no colonies. All the reagents and protocols work fine for my old strains.
Why could this be? I picked a second clone of the knockout and it behaves the same: No colonies from electroporations.
The KO strains seem to grow pretty normally in broth. I don't see a growth defect.
I used hygromycin selection to make the KO and hygromycin to select for electroporation transformants. Could that cause problems? The deletion mutant should have completely lost the resistance cassette, and, indeed, when I put my KO strain in hygromyicin selection it does not survive -- consistent with successful unmarked deletion.
Fig 2 shows the genetics and Fig 3 shows the workflow. Section 3.5 shows the steps.
Stable extracellular enzyme activities are associated with soil colloids and persist even in harsh environments that would limit intracellular microbiological activity. Thus, only strictly intracellular enzyme activities can truly reflect microbial activity because the contribution of free extracellular enzyme released by active microbial cells is negligible; indeed, these enzymes are shortlived because they are degraded by proteases unless they are adsorbed by clays.
I want to predict operons on entire bacterial genomes (already annotated). I used to use operon-mapper ( https://academic.oup.com/bioinformatics/article/34/23/4118/5040321), which is great but it has been "under maintenance" for weeks now and I really need one now.
MicrobesOnline doesn't support private genome hosting anymore and I think that the DOOR website is now down.
If you know any other tool, online or not, that would allow the genome-wide prediction of operons in complete, annotated bacterial genomes, that would be of great help to me.
I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field.
It has been kept alive for several months in a bioreactor.
I want to pull some of this bacteria out of the bioreactor and put it in a fridge for several months to examine later and possibly use it to re-seed a different bioreactor.
What would be the best way to preserve a heterogeneous culture of nitrifying bacteria?
(As a note, this culture contains ammonia oxidizing, nitrite oxidizing, AND heterotrophic bacteria)
I need your help on how to calculate CFU/g soil. I would greatly appreciate if you could check if my calculation is correct.
5 g of soil was placed into 50 mL water. The solution was serial diluted 10x (1/10). 0.1 mL of the dilution was spread onto the agar plate.
30 bacterial colonies were obtained.
5 g of soil was placed in 50 mL water so I have 100 mg soil in 1 mL of water.
With a dilution of 1/10, I have 10 mg of soil in 1mL of water
Next, I spread 0.1 mL of this dilution -> 1 mg of soil
I have obtained 30 CFU so I have 30 CFU on 1 mg of soil. Consequently, I have 30 000 CFU on 1 g of soil
Is this correct?
Thanks in advance.
Vertigo is a condition that can make it feel like you or your surroundings are spinning, sometimes leading to a loss of balance, according to the U.S. National Library of Medicine.
Coronavirus 2019 or COVID-19 is a novel entity which had led to many challenges among physicians due to its rapidly evolving nature. Vertigo or dizziness has recently been described as a clinical manifestation of COVID-19.
So, Are dizziness and vertigo COVID-19 Symptoms? and why?
Please note that the data is excel sheet is not my real data and my intention behind attaching the excel file is only to give our feedback community a clearer idea of how my data is expressed. This is the reason I have not run a two way ANOVA for this hypothetical data values. However, I have run a two-way ANOVA for my actual dataset.
Let's suppose I have a dataset for 'three types of vehicles (let's call them A,B,C), three types of tires (let's call them P,Q,R), and I measure the speed of each vehicle for each type of the tire. I record total 6 values for speed for each combination of vehicle and tire (Please view the excel file so as to get a clearer idea of how my data is expressed). I run a two-way ANOVA and I realize that my data does not follow homogeneity of variance. However, it is normally distributed and there are no outliers.
Then, I transform my data to log values however, I encounter the same issue (i.e. homogeneity of variance violated, data is however, normally distributed and no outliers).
So, my question is, do we have a non-parametric test that I can run instead of two-way ANOVA to analyze my data and obtain similar parameters in the output as a two-way ANOVA (such as mainly telling me the significant differences if there are any between "tires P for vehicle B" and "tire Q for vehicle C")?
Software I use: Excel, SPSS.
the study of microorganisms is called microbiology
Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental research on the biochemistry, physiology, cell biology, ecology, evolution and clinical aspects of microorganisms, including the host response to these agents.
Questions regarding biotechnology and microbiological and bio chemistry DEPARTMENT practically project working my contacts number is 8248413604
In the attached picture, you can see the primary RPE cells contaminated with small motile rode shape bacteria in 40 magnification microscope image. i cultured the extracted cells in Miller medium included 2%pen-strep and 10%pig serum. the bacterial contamination can be removed(not always) if i change medium every day . If not, under microscope i can see these bacteria which are motile and are moving in the living cells and in the space between them and after some day the cells all will be dead .
I think they are not mycoplasma. because I see them individually under 40 magnification microscope, how is your opinion?
can some body help me
1)which antibiotics can remove them?
2)what kind of microbes are there?
We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We have followed four papers to make agar medium with skim milk (with 5% NaCl). But every time the media got clotted after autoclave.
What is the reason and solution for this problem? Can anyone suggest anything?
We have followed these papers:
1. Babavalian, H., Amoozegar, M.A., Pourbabaee, A.A. et al. Isolation and identification of moderately halophilic bacteria producing hydrolytic enzymes from the largest hypersaline playa in Iran. Microbiology 82, 466–474 (2013). https://doi.org/10.1134/S0026261713040176
2. Adinarayana, K., Ellaiah, P. & Prasad, D.S. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS PharmSciTech 4, 440–448 (2003). https://doi.org/10.1208/pt040456
3. Wery, N., Gerike, U., Sharman, A., Chaudhuri, J. B., Hough, D. W., & Danson, M. J. (2003). Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil. Applied and environmental microbiology, 69(3), 1457–1464. https://doi.org/10.1128/aem.69.3.1457-1464.2003
4. Rydén, A. C., Lindberg, M., & Philipson, L. (1973). Isolation and characterization of two protease-producing mutants from Staphylococcus aureus. Journal of bacteriology, 116(1), 25–32. https://doi.org/10.1128/JB.116.1.25-32.1973
Microbiologist’s opinion needed
Short version: We want to study microbiologically influenced corrosion in simulated seawater. How do we cultivate marine bacteria in the laboratory?
my mentor and I have been studying electrochemical behavior of metals and alloys in simulated acid rain. Since we came across alloys (steel and bronze) used in the shipbuilding industry we thought about changing the media for seawater. If we do so, we are obliged to take into consideration microorganisms living in the seawater and participating in direct corrosion processes through the formation of complex bioflms.
We were wondering, is there any “easy” way, or to say beginner cultures to start with? We’re aware of a hustle it may be to get the experiment and the conditions right or take care not to contaminate the working station. What interests us the most are bacteria or algae from Central or Southern Adriatic. We may also order a MIC culture from abroad (sulfate-reducing bacteria is the most popular one atm). Either way, we don’t have confidence in growing them in the laboratory or the right knowledge to set up conditions for the experiment. Thought someone would share some insights and help us plan ahead. Should we try getting pure culture from natural seawater sample or just make an online purchase?
We’re only interested in examining corrosion parameters on the metal surface, but if anyone finds it interesting and would like to contribute with their knowledge more, we’d be more than happy to broaden our horizons and collaborate. I have a Master’s degree in Biology and Chemistry and would love to explore the dynamics of processes and interactions between microorganisms and metal surfaces more.
Lastly, is bacterial culturing even worth trying if we don’t have a Microbiology expert by our side?
Looking forward to the answers,
please feel free to message me if you want to discuss more.
Thank you for your time,
I am trying to create a graph on excel to express -log10 reduction of treatment groups and CFU spores using the equation -log10(final concentration/initial). However, one of my groups does not have a control to compare to.
How do i overcome this? Analysing data given to me
I've been trying to recover some bacterial cells by streaking them on agar plates, without success.
The stock has been kept in good conditions. But, there is a white pellet at the end of the glycerol tube, which I'm not actually sure whether it's the bacterial cells or not.
Do bacterial cells form pellets at the end of the glycerol stock tube?
Is there any way to recover them from the glycerol stock?
I am having trouble figuring out where to obtain Streptomyces lividans strains that are commonly used for enzyme production. Specifically I am searching for the following strains: S. lividans TK24, 1326 and/or GSAL1 . They don't seem to be available from ATCC. Does anyone have any suggestions? Thanks in advance!
I am calculating intraparticle diffusion for adsorption studies . For calculating diffusion co efficient we need to know the radius of the adsorbent material .My material after activating i sieved and found its below 500 micrometre . I need to know if this is the radius or we have to calculate with any other methods.
I'm working with cheese whey as an alternative culture medium. So far, the sterilization method that I use involves the precipitation of insoluble compounds (including proteins) by lowering the pH and heating for an extended period of time. Autoclave conditions are too extreme for the media and results in a slightly burnt color and smell.
I am wondering if there is an existing protocol that would allow sterilization of the media without precipitating the proteins.
Thank you for your response!